Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 500827 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#500827, RRID:AB_2562311
- Product name
- Brilliant Violet 605™ anti-human IL-4
- Antibody type
- Monoclonal
- Antigen
- CHO-expressed, recombinant human IL-4
- Reactivity
- Human, Porcine
- Host
- Rat
- Conjugate
- Orange dye
- Isotype
- IgG
- Antibody clone number
- MP4-25D2
- Vial size
- 25 tests
- Storage
- 4° C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- PMA+ ionomycin-stimulated human peripheral blood lymphocytes (6 hours, in the presence of monensin) were surface stained with CD3 APC, fixed, permeabilized and then stained with IL-4 (clone MP4-25D2) Brilliant Violet 605™ (top) or rat IgG1, κ Brilliant Violet 605™ isotype control (bottom).
- Conjugate
- Yellow dye