Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C154120 - Provider product page
- Provider
- LSBio
- Product name
- IL4 Antibody (Biotin) LS-C154120
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Biotin Conjugated Rabbit anti-IL-4 Antibody. Lane 1: Human IL-4. Lane 2: none. Load: 50 ng per lane. Primary antibody: IL-4 Antibody Biotin Conjugated at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-4. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.