Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF584 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects cotton rat IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat IL-4 is observed, 5% cross-reactivity with recombinant mouse IL-4 is observed, and less than 1% cross-reactivity with recombinant human IL-4 and recombinant porcine IL-4 is observed.
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Cotton Rat IL-4 Antibody. Recombinant Cotton Rat IL-4 (Catalog # 584-R4) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-4 (10 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-4 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF584). The ND50 is typically 0.075-0.3 µg/mL in the presence of PHA (10 µg/mL).