Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-404-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-4 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects mouse IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat IL-4 and no cross-reactivity with recombinant human IL-4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The spleen is the site where mast cells are induced in the development of food allergy.
The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
Suppression of autoimmune inflammation of the central nervous system by interleukin 10 secreted by interleukin 27-stimulated T cells.
Toyoshima S, Wakamatsu E, Ishida Y, Obata Y, Kurashima Y, Kiyono H, Abe R
International immunology 2017 Jan 1;29(1):31-45
International immunology 2017 Jan 1;29(1):31-45
The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
El-Behi M, Ciric B, Dai H, Yan Y, Cullimore M, Safavi F, Zhang GX, Dittel BN, Rostami A
Nature immunology 2011 Jun;12(6):568-75
Nature immunology 2011 Jun;12(6):568-75
Suppression of autoimmune inflammation of the central nervous system by interleukin 10 secreted by interleukin 27-stimulated T cells.
Fitzgerald DC, Zhang GX, El-Behi M, Fonseca-Kelly Z, Li H, Yu S, Saris CJ, Gran B, Ciric B, Rostami A
Nature immunology 2007 Dec;8(12):1372-9
Nature immunology 2007 Dec;8(12):1372-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Mouse IL-4 Antibody. Recombinant Mouse IL-4 (Catalog # 404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-4 Polyclonal Antibody (Catalog # AB-404-NA). The ND50 is typically 3-5 µg/mL.