Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF-504-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat IL-4 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant cotton rat IL-4 and recombinant mouse IL-4 is observed, and less than 1% cross-reactivity with recombinant human IL-4, recombinant canine IL-4, and recombinant porcine IL-4 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Interleukin-4 and Interleukin-13 Exacerbate Neurotoxicity of Prothrombin Kringle-2 in Cortex In Vivo via Oxidative Stress.
Endogenous expression of interleukin-4 regulates macrophage activation and confines cavity formation after traumatic spinal cord injury.
Jeong JY, Chung YC, Jin BK
International journal of molecular sciences 2019 Apr 19;20(8)
International journal of molecular sciences 2019 Apr 19;20(8)
Endogenous expression of interleukin-4 regulates macrophage activation and confines cavity formation after traumatic spinal cord injury.
Lee SI, Jeong SR, Kang YM, Han DH, Jin BK, Namgung U, Kim BG
Journal of neuroscience research 2010 Aug 15;88(11):2409-19
Journal of neuroscience research 2010 Aug 15;88(11):2409-19
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Rat IL-4 Antibody. Recombinant Rat IL-4 (Catalog # 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-4 (4 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA). The ND50 is typically 0.05-015 µg/mL in the presence of PHA (10 µg/mL).