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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB5041-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant rat IL-4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human IL-4, recombinant mouse IL-4, recombinant feline IL-4, recombinant canine IL-4, recombinant porcine IL-4, recombinant cotton rat IL-4, or recombinant mouse IL-13 is observed.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Rat IL-4 Antibody. Recombinant Rat IL-4 (Catalog # 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Rat IL-4 (4 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Rat IL-4 Monoclonal Antibody (Catalog # MAB5041). The ND50 is typically 0.8-4.0 µg/mL in the presence of PHA (10 µg/mL).
