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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB984 - Provider product page
- Provider
- R&D Systems
- Product name
- Feline IL-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects feline IL-4 in ELISAs and Western blots. In Western blots, no cross-reactivity with human, mouse, or canine IL-4 is observed.
- Reactivity
- Feline
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P55030- Isotype
- IgG
- Antibody clone number
- 159638
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Feline IL-4 Antibody. Recom-binant Feline IL-4 (Catalog # 984-FL) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Feline IL-4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-4 Mono-clonal Antibody (Catalog # MAB984). The ND50 is typically 1-4 µg/mL.
