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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB404-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-4 in ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P07750- Isotype
- IgG
- Antibody clone number
- 30340
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis.
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
ROR inverse agonist suppresses insulitis and prevents hyperglycemia in a mouse model of type 1 diabetes.
TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.
Basophils control T-cell responses and limit disease activity in experimental murine colitis.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
CCL25 induces α₄β₇ integrin-dependent migration of IL-17⁺ γδ T lymphocytes during an allergic reaction.
The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator.
Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Nam Y, Jung SM, Rim YA, Jung H, Lee K, Park N, Kim J, Jang Y, Park YB, Park SH, Ju JH
PloS one 2018;13(6):e0198740
PloS one 2018;13(6):e0198740
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
ROR inverse agonist suppresses insulitis and prevents hyperglycemia in a mouse model of type 1 diabetes.
Solt LA, Banerjee S, Campbell S, Kamenecka TM, Burris TP
Endocrinology 2015 Mar;156(3):869-81
Endocrinology 2015 Mar;156(3):869-81
TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.
Reba SM, Li Q, Onwuzulike S, Ding X, Karim AF, Hernandez Y, Fulton SA, Harding CV, Lancioni CL, Nagy N, Rodriguez ME, Wearsch PA, Rojas RE
European journal of immunology 2014 May;44(5):1410-21
European journal of immunology 2014 May;44(5):1410-21
Basophils control T-cell responses and limit disease activity in experimental murine colitis.
Gomez MR, Talke Y, Hofmann C, Ketelsen I, Hermann F, Reich B, Goebel N, Schmidbauer K, Dunger N, Brühl H, Renner K, Syed SN, Mack M
Mucosal immunology 2014 Jan;7(1):188-99
Mucosal immunology 2014 Jan;7(1):188-99
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
CCL25 induces α₄β₇ integrin-dependent migration of IL-17⁺ γδ T lymphocytes during an allergic reaction.
Costa MF, Bornstein VU, Candéa AL, Henriques-Pons A, Henriques MG, Penido C
European journal of immunology 2012 May;42(5):1250-60
European journal of immunology 2012 May;42(5):1250-60
The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
Jin W, Qi S, Luo H
BMC immunology 2011 Dec 19;12:68
BMC immunology 2011 Dec 19;12:68
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
Microbes and infection 2009 Apr;11(4):524-30
Microbes and infection 2009 Apr;11(4):524-30
TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4089-97
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4089-97
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology 2008 Oct;125(2):272-9
Immunology 2008 Oct;125(2):272-9
Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator.
Chiang YM, Chang CL, Chang SL, Yang WC, Shyur LF
Journal of ethnopharmacology 2007 Apr 4;110(3):532-8
Journal of ethnopharmacology 2007 Apr 4;110(3):532-8
Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
Wen H, Hogaboam CM, Gauldie J, Kunkel SL
The American journal of pathology 2006 Jun;168(6):1940-50
The American journal of pathology 2006 Jun;168(6):1940-50
Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
Bednarski JJ, Warner RE, Rao T, Leonetti F, Yung R, Richardson BC, Johnson KJ, Ellman JA, Opipari AW Jr, Glick GD
Arthritis and rheumatism 2003 Mar;48(3):757-66
Arthritis and rheumatism 2003 Mar;48(3):757-66
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
The American journal of pathology 2002 Apr;160(4):1353-60
The American journal of pathology 2002 Apr;160(4):1353-60
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Mouse IL-4 Antibody. Recombinant Mouse IL-4 (Catalog # 404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-4 Monoclonal Antibody (Catalog # MAB404). The ND50 is typically 0.1-0.6 µg/mL.
