Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB304-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-4 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL-4.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P05112.1
- Isotype
- IgG
- Antibody clone number
- 3007
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1β-stimulated production of chemokines and matrix-degrading enzymes in vitro.
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway.
Ranzani V, Rossetti G, Panzeri I, Arrigoni A, Bonnal RJ, Curti S, Gruarin P, Provasi E, Sugliano E, Marconi M, De Francesco R, Geginat J, Bodega B, Abrignani S, Pagani M
Nature immunology 2015 Mar;16(3):318-325
Nature immunology 2015 Mar;16(3):318-325
Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1β-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzì RM, Meliconi R
PloS one 2014;9(5):e96925
PloS one 2014;9(5):e96925
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4880-8
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4880-8
Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
The Journal of allergy and clinical immunology 2007 Nov;120(5):1118-25
The Journal of allergy and clinical immunology 2007 Nov;120(5):1118-25
Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway.
Aoudjehane L, Podevin P, Scatton O, Jaffray P, Dusanter-Fourt I, Feldmann G, Massault PP, Grira L, Bringuier A, Dousset B, Chouzenoux S, Soubrane O, Calmus Y, Conti F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 May;21(7):1433-44
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 May;21(7):1433-44
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-4 in Human PBMCs. IL-4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-4 and Neutralization by Human IL-4 Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB304). The ND50 is typically 0.03-0.1 µg/mL.