Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB2469 - Provider product page
- Provider
- R&D Systems
- Product name
- Bovine IL-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects bovine IL-4 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant porcine, canine, rhesus, cotton rat, equine, or rabbit IL-4 is observed and no cross-reactivity with recombinant human, mouse, rat, or feline IL-4 is observed.
- Reactivity
- Bovine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P30367.2- Isotype
- IgG
- Antibody clone number
- 701214
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Bovine IL-4 by Western Blot. Western blot shows Recombinant Bovine IL-4 (Catalog # 2469-BL) (10 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-4 in Bovine PBMCs. IL-4 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) stimulated with concanavalin A using Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
