13-7048-85

antibody from Invitrogen Antibodies

Targeting: IL4 BCGF-1, BCGF1, BSF1, IL-4, MGC79402

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13-7048-85

Provider

Invitrogen Antibodies

Product name

IL-4 Monoclonal Antibody (MP4-25D2), Biotin, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells. Applications Reported: The MP4-25D2 antibody has been reported for use in detection of human IL-4 by ELISA and for intracellular staining for flow cytometry. Applications Tested: The MP4-25D2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the purified 8D4-8 (14-7049) antibody for capture and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Rat

Conjugate

Biotin

Isotype

IgG

Antibody clone number

MP4-25D2

Vial size

500 µg

Concentration

0.5 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Fasting-induced FOXO4 blunts human CD4(+) T helper cell responsiveness.

Han K, Singh K, Rodman MJ, Hassanzadeh S, Wu K, Nguyen A, Huffstutler RD, Seifuddin F, Dagur PK, Saxena A, McCoy JP, Chen J, Biancotto A, Stagliano KER, Teague HL, Mehta NN, Pirooznia M, Sack MN
Nature metabolism 2021 Mar;3(3):318-326

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A molecular signature of preclinical rheumatoid arthritis triggered by dysregulated PTPN22.

Chang HH, Liu GY, Dwivedi N, Sun B, Okamoto Y, Kinslow JD, Deane KD, Demoruelle MK, Norris JM, Thompson PR, Sparks JA, Rao DA, Karlson EW, Hung HC, Holers VM, Ho IC
JCI insight 2016 Oct 20;1(17):e90045

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Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.

Luchansky MS, Bailey RC
Journal of the American Chemical Society 2011 Dec 21;133(50):20500-6

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 3. Fasting-induced FOXO4 Effects. a , FOXO4 transcription network and its targets identified by GeneMANIA analysis. The 24-hr. fasting DE genes (circular nodes) show fold increased (blue circles) or decreased (red circles) expression relative to refeeding levels. Validation of target relative genes transcript levels by qRT-PCR in PBMCs (bar plots mean +- SEM with mean values of 3-4 replicates from n=14 subjects, two-sided, paired Student's t-test). b , Concurrent analysis using the Find Individual Motif Occurrences (FIMO) tool identified the FOXO4 binding motif in the promoter regions of a significant proportion of fasted DE upregulated genes (fisher exact test for FOXO4 motif enrichment significance, p=0.049). c , Representative protein blots from 3 subjects (left panel) and quantitative changes (right panel) of FOXO4 levels in CD4 + T cells from the 3 caloric-load states. (Bar graph mean +- SEM, n=7 subjects, two-sided, paired Student's t-test). d-e , Cytokine release of IFNgamma, IL-4 and IL-17 following FOXO4 knockdown (KD) and overexpression in activated CD4 + T cells. The cytokine release of KD cells measured (n=5 separate experiment (d) ) or from FOXO4-overexpressed cells (n=10 separate experiments (e) ) using two-sided, paired Student's t-test analysis. The dot and line plots show the mean value of each subject and the values represent the average of duplicate experiments. Statistical analysis using two-sided, paired Wilcoxon test. f-g, Representative protein b

Conjugate

Biotin