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- Antigen structure
- References [14]
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- Product number
- 14-7048-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-4 Monoclonal Antibody (MP4-25D2), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells. Applications Reported: MP4-25D2 has been reported for use in neutralizing IL-4 binding and biological activity. (Please use Functional Grade purified MP4-25D2, Product # 16-7048 , in functional assays.) Please refer to the supporting protocols for more information. Applications Tested: The purified MP4-25D2 can be used for blocking of fluorochrome conjugated MP4-25D2 in intracellular flow cytometry experiments to serve as specificity control. This biotinylated format for this lot of MP4-25D2 has been tested by sandwich ELISA for detection of human IL-4 in combination with 8D4-8 and can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- MP4-25D2
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Fasting-induced FOXO4 blunts human CD4(+) T helper cell responsiveness.
Reciprocal regulation of Th2 and Th17 cells by PAD2-mediated citrullination.
IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation.
An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells.
Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.
IFN-γ-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.
Human CD1c⁺ myeloid dendritic cells acquire a high level of retinoic acid-producing capacity in response to vitamin D₃.
Intercellular adhesion molecule 1 mediates migration of Th1 and Th17 cells across human retinal vascular endothelium.
C3a modulates IL-1β secretion in human monocytes by regulating ATP efflux and subsequent NLRP3 inflammasome activation.
Dominant Th2 differentiation of human regulatory T cells upon loss of FOXP3 expression.
CD137 ligand signaling induces human monocyte to dendritic cell differentiation.
De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.
Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies.
Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Han K, Singh K, Rodman MJ, Hassanzadeh S, Wu K, Nguyen A, Huffstutler RD, Seifuddin F, Dagur PK, Saxena A, McCoy JP, Chen J, Biancotto A, Stagliano KER, Teague HL, Mehta NN, Pirooznia M, Sack MN
Nature metabolism 2021 Mar;3(3):318-326
Nature metabolism 2021 Mar;3(3):318-326
Reciprocal regulation of Th2 and Th17 cells by PAD2-mediated citrullination.
Sun B, Chang HH, Salinger A, Tomita B, Bawadekar M, Holmes CL, Shelef MA, Weerapana E, Thompson PR, Ho IC
JCI insight 2019 Nov 14;4(22)
JCI insight 2019 Nov 14;4(22)
IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation.
Zhao M, Tan Y, Peng Q, Huang C, Guo Y, Liang G, Zhu B, Huang Y, Liu A, Wang Z, Li M, Gao X, Wu R, Wu H, Long H, Lu Q
Nature communications 2018 Feb 8;9(1):583
Nature communications 2018 Feb 8;9(1):583
An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells.
Massoud AH, Charbonnier LM, Lopez D, Pellegrini M, Phipatanakul W, Chatila TA
Nature medicine 2016 Sep;22(9):1013-22
Nature medicine 2016 Sep;22(9):1013-22
Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.
Deng N, Weaver JM, Mosmann TR
PloS one 2014;9(5):e95986
PloS one 2014;9(5):e95986
IFN-γ-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.
Svajger U, Obermajer N, Jeras M
Journal of leukocyte biology 2014 Jan;95(1):33-46
Journal of leukocyte biology 2014 Jan;95(1):33-46
Human CD1c⁺ myeloid dendritic cells acquire a high level of retinoic acid-producing capacity in response to vitamin D₃.
Sato T, Kitawaki T, Fujita H, Iwata M, Iyoda T, Inaba K, Ohteki T, Hasegawa S, Kawada K, Sakai Y, Ikeuchi H, Nakase H, Niwa A, Takaori-Kondo A, Kadowaki N
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):3152-60
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):3152-60
Intercellular adhesion molecule 1 mediates migration of Th1 and Th17 cells across human retinal vascular endothelium.
Bharadwaj AS, Schewitz-Bowers LP, Wei L, Lee RW, Smith JR
Investigative ophthalmology & visual science 2013 Oct 23;54(10):6917-25
Investigative ophthalmology & visual science 2013 Oct 23;54(10):6917-25
C3a modulates IL-1β secretion in human monocytes by regulating ATP efflux and subsequent NLRP3 inflammasome activation.
Asgari E, Le Friec G, Yamamoto H, Perucha E, Sacks SS, Köhl J, Cook HT, Kemper C
Blood 2013 Nov 14;122(20):3473-81
Blood 2013 Nov 14;122(20):3473-81
Dominant Th2 differentiation of human regulatory T cells upon loss of FOXP3 expression.
Hansmann L, Schmidl C, Kett J, Steger L, Andreesen R, Hoffmann P, Rehli M, Edinger M
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1275-82
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1275-82
CD137 ligand signaling induces human monocyte to dendritic cell differentiation.
Kwajah M M S, Schwarz H
European journal of immunology 2010 Jul;40(7):1938-49
European journal of immunology 2010 Jul;40(7):1938-49
De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.
Wang J, Huizinga TW, Toes RE
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 15;183(6):4119-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 15;183(6):4119-26
Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies.
Ramanathan L, Ingram R, Sullivan L, Greenberg R, Reim R, Trotta PP, Le HV
Biochemistry 1993 Apr 13;32(14):3549-56
Biochemistry 1993 Apr 13;32(14):3549-56
Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE
Immunological reviews 1992 Jun;127:5-24
Immunological reviews 1992 Jun;127:5-24
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- Invitrogen Antibodies (provider)
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3. Fasting-induced FOXO4 Effects. a , FOXO4 transcription network and its targets identified by GeneMANIA analysis. The 24-hr. fasting DE genes (circular nodes) show fold increased (blue circles) or decreased (red circles) expression relative to refeeding levels. Validation of target relative genes transcript levels by qRT-PCR in PBMCs (bar plots mean +- SEM with mean values of 3-4 replicates from n=14 subjects, two-sided, paired Student's t-test). b , Concurrent analysis using the Find Individual Motif Occurrences (FIMO) tool identified the FOXO4 binding motif in the promoter regions of a significant proportion of fasted DE upregulated genes (fisher exact test for FOXO4 motif enrichment significance, p=0.049). c , Representative protein blots from 3 subjects (left panel) and quantitative changes (right panel) of FOXO4 levels in CD4 + T cells from the 3 caloric-load states. (Bar graph mean +- SEM, n=7 subjects, two-sided, paired Student's t-test). d-e , Cytokine release of IFNgamma, IL-4 and IL-17 following FOXO4 knockdown (KD) and overexpression in activated CD4 + T cells. The cytokine release of KD cells measured (n=5 separate experiment (d) ) or from FOXO4-overexpressed cells (n=10 separate experiments (e) ) using two-sided, paired Student's t-test analysis. The dot and line plots show the mean value of each subject and the values represent the average of duplicate experiments. Statistical analysis using two-sided, paired Wilcoxon test. f-g, Representative protein b
