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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- 53-7049-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-4 Monoclonal Antibody (8D4-8), Alexa Fluor 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells. Applications Reported: This 8D4-8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 8D4-8 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 8D4-8
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.
Arginine kinase Pen m 2 as an important shrimp allergen recognized by TH2 cells.
Zhong F, Zhou N, Wu K, Guo Y, Tan W, Zhang H, Zhang X, Geng G, Pan T, Luo H, Zhang Y, Xu Z, Liu J, Liu B, Gao W, Liu C, Ren L, Li J, Zhou J, Zhang H
Nucleic acids research 2015 Dec 2;43(21):10474-91
Nucleic acids research 2015 Dec 2;43(21):10474-91
Arginine kinase Pen m 2 as an important shrimp allergen recognized by TH2 cells.
Renand A, Newbrough S, Wambre E, DeLong JH, Robinson D, Kwok WW
The Journal of allergy and clinical immunology 2014 Dec;134(6):1456-1459.e7
The Journal of allergy and clinical immunology 2014 Dec;134(6):1456-1459.e7
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0048-42) and IL-4 Monoclonal Antibody, Alexa Fluor 488. Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye
