500712

antibody from BioLegend

Targeting: IL4 BCGF-1, BCGF1, BSF1, IL-4, MGC79402

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

500712

Provider

BioLegend

Proper citation

BioLegend Cat#500712, RRID:AB_1877137

Product name

Alexa Fluor® 647 anti-human IL-4 [8D4-8]

Antibody type

Monoclonal

Antigen

Yeast-expressed, recombinant human IL-4

Description

IL-4 is a pleiotropic cytokine that is produced by activated T cells mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. The 8D4-8 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

100 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection.

Gong G, Shao L, Wang Y, Chen CY, Huang D, Yao S, Zhan X, Sicard H, Wang R, Chen ZW
Blood 2009 Jan 22;113(4):837-45

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes surface stained with CD3 PE, then intracellular stained with 8D4-8 Alexa Fluor® 647 (top), bottom data shows the 8D4-8 Alexa Fluor® 647 staining can be specifically blocked by pre-incubation with purified 8D4-8 antibody

Conjugate

Red dye