66142-1-Ig

antibody from Proteintech Group

Targeting: IL4 BCGF-1, BCGF1, BSF1, IL-4, MGC79402

Provider product page for 66142-1-Ig

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [46]
Comments [0]
Validations
Western blot [2]

Submit

Product number: 66142-1-Ig - Provider product page
Provider: Proteintech Group
Product name: IL-4 antibody
Antibody type: Monoclonal
Description: IL-4 antibody (Cat. #66142-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, pig and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Porcine
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 1G2C5
Vial size: 20ul, 150ul

IL4 protein structure - 66142-1-Ig shown in red.

Submitted references Resatorvid (TAK-242) Ameliorates Ulcerative Colitis by Modulating Macrophage Polarization and T Helper Cell Balance via TLR4/JAK2/STAT3 Signaling Pathway.

Huang X, Lin R, Liu H, Dai M, Guo J, Hui W, Liu W, Haerken M, Zheng R, Yushanjiang T, Gao F
Inflammation 2024 May 18;

Artesunate-loaded thermosensitive chitosan hydrogel promotes osteogenesis of maxillary tooth extraction through regulating T lymphocytes in type 2 diabetic rats.

Luo J, Liang C, Chen K, Zeng K, Bai R, Tang C, Li J, Nong X
BMC oral health 2024 Mar 20;24(1):356

Myeloid-derived growth factor ameliorates dextran sodium sulfate-induced colitis by regulating macrophage polarization.

Yang Y, Zhao C, Yang Z, Du C, Chang Z, Wen X, Zhang X, Liu Y, Hu L, Gao Z
Journal of molecular medicine (Berlin, Germany) 2024 Jul;102(7):875-886

Cosentyx alleviates psoriasis-induced podocyte injury by inhibiting the tlr/nf-κb signaling pathway.

Aixue W, Feng W, Huanhuan Z, Xixing M, Yanling L
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 2024 Feb;30(2):e13562

Sodium-glucose co-transporter 2 inhibitor canagliflozin modulates myocardial metabolism and inflammation in a swine model for chronic myocardial ischemia.

Harris DD, Sabe SA, Xu CM, Sabra M, Broadwin M, Malhotra A, Li JW, Abid MR, Sellke FW
Surgery 2024 Feb;175(2):265-270

Alleviation of allergic asthma by rosmarinic acid via gut-lung axis.

Guo HH, Han YX, Rong XJ, Shen Z, Shen HR, Kong LF, Guo YD, Li JZ, Xu B, Gao TL, Wang LL, Tie C, Jiang JD
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Apr;126:155470

Favipiravir ameliorates bleomycin-induced pulmonary fibrosis by reprogramming M1/M2 macrophage polarization.

Zhang R, Jiang Q, Gao S, Zhang H, Xia Q, Liu B, Zhu J, Jiang H, Zhao R, Dong H, Li X, Zhang Y, Yang C, Gu X, Sun L, Zhou H
International immunopharmacology 2024 Apr 20;131:111774

Dupilumab improve acquired reactive perforating collagenosis characterized by type 2 inflammation.

Liu B, Wu Y, Wu X, Zhong X, Xue R, Zhang Z
Frontiers in immunology 2023;14:1240262

Immunity and reproduction protective effects of Chitosan Oligosaccharides in Cyclophosphamide/Busulfan-induced premature ovarian failure model mice.

Li X, Ye H, Su T, Hu C, Huang Y, Fu X, Zhong Z, Du X, Zheng Y
Frontiers in immunology 2023;14:1185921

Antimicrobial peptide-modified AIE visual composite wound dressing for promoting rapid healing of infected wounds.

Chen Y, Qian H, Peng D, Jiang Y, Liu Q, Tan Y, Feng L, Cheng B, Li G
Frontiers in bioengineering and biotechnology 2023;11:1338172

The effect of acupuncture on lateral habenular nucleus and intestinal microflora in depression model rats.

Chen W, Chen Y, Aslam MS, Shen J, Tong T, Yan S, Cheng W, Huang Z, Li J, Liu S, Li J, Zeng J, Li M, You Z, Meng X
Behavioural brain research 2023 Oct 18;455:114627

PCSK9 inhibition ameliorates experimental autoimmune myocarditis by reducing Th17 cell differentiation through LDLR/STAT-3/ROR-γt pathway.

Yu M, Tang W, Liang W, Xie B, Gao R, Ding P, Gu X, Wang M, Wen S, Sun P
International immunopharmacology 2023 Nov;124(Pt B):110962

Expression pattern analysis of m6A regulators reveals IGF2BP3 as a key modulator in osteoarthritis synovial macrophages.

Lu Y, Zhang H, Pan H, Zhang Z, Zeng H, Xie H, Yin J, Tang W, Lin R, Zeng C, Cai D
Journal of translational medicine 2023 May 22;21(1):339

Investigation of macrophage polarization in herniated nucleus pulposus of patients with lumbar intervertebral disc herniation.

Li XC, Luo SJ, Wu F, Mu QC, Yang JH, Jiang C, Wang W, Zhou TL, Qin TD, Tan RX, Jian-Li, Huang CM, Wang MS, Bai XC
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2023 Jun;41(6):1335-1347

Ketogenic diet attenuates neuroinflammation and induces conversion of M1 microglia to M2 in an EAE model of multiple sclerosis by regulating the NF-κB/NLRP3 pathway and inhibiting HDAC3 and P2X7R activation.

Sun W, Wang Q, Zhang R, Zhang N
Food & function 2023 Jul 31;14(15):7247-7269

Induced Overexpression of B Cell-Activating Factor by Triiodothyronine Results in Abnormal B Cell Differentiation in Mice.

Liu S, Li GQ, Gu QW, Wang J, Sun Q, Gu WS, Mao XM
Cell transplantation 2023 Jan-Dec;32:9636897231204075

Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent Preeclampsia.

Liu H, Yu L, Ding Y, Peng M, Deng Y
Cell transplantation 2023 Jan-Dec;32:9636897221145682

Intrahepatic eosinophilic proliferative phlebitis in Japanese black cattle indicate allergies involving mast cell tryptase-dependent activation.

Konnai M, Takahashi K, Machida Y, Michishita M, Ohkusu-Tsukada K
Frontiers in veterinary science 2022;9:972180

Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing.

Gong X, Liu L, Li X, Xiong J, Xu J, Mao D, Liu L
Frontiers in nutrition 2022;9:1028459

Baicalin Attenuates Continuous Activation of β-Catenin Induced by Lipopolysaccharide (LPS) and Depression Complicated by Infertility in Male Rats.

Fan R, Dai F, Zhang C, Zhou L, Qiu X, Li Y
Evidence-based complementary and alternative medicine : eCAM 2022;2022:2112359

IL-4 and CCR7 play an important role in the development of keloids in patients with a family history.

Shan M, Liu H, Hao Y, Meng T, Feng C, Song K, Wang Y
American journal of translational research 2022;14(5):3381-3394

Intravenous AAV9 administration results in safe and widespread distribution of transgene in the brain of mini-pig.

Lin Y, Li C, Wang W, Li J, Huang C, Zheng X, Liu Z, Song X, Chen Y, Gao J, Wu J, Wu J, Tu Z, Lai L, Li XJ, Li S, Yan S
Frontiers in cell and developmental biology 2022;10:1115348

Mesoporous silica nanoparticle-modified electrode arrays of cochlear implants for delivery of siRNA-TGFβ1 into the inner ear.

Chen A, Chen Y, Liu S, Ma D, Tang J, Zhang H
Colloids and surfaces. B, Biointerfaces 2022 Oct;218:112753

Schisandra chinensis Lignans Exert Antidepressant Effects by Promoting BV2 Microglia Polarization toward the M2 Phenotype through the Activation of the Cannabinoid Receptor Type-2-Signal Transducer and Activator of Transcription 6 Pathway.

Wang J, Zhang G, Yang Y, Zhang X, Shi K, Zhang X, Yan T, Jia Y
Journal of agricultural and food chemistry 2022 Nov 9;70(44):14157-14169

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

Fan TW, Daneshmandi S, Cassel TA, Uddin MB, Sledziona J, Thompson PT, Lin P, Higashi RM, Lane AN
Journal of immunology (Baltimore, Md. : 1950) 2022 Nov 1;209(9):1674-1690

Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia.

Tan Z, Yang G, Qiu J, Yan W, Liu Y, Ma Z, Li J, Liu J, Shan N
Molecular neurobiology 2022 May;59(5):3140-3158

Interleukin-4 activates the PI3K/AKT signaling to promote apoptosis and inhibit the proliferation of granulosa cells.

Han Y, Yao R, Yang Z, Li S, Meng W, Zhang Y, Zhang Y, Luo H
Experimental cell research 2022 Mar 1;412(1):113002

Chitooligosaccharides alleviate hepatic fibrosis by regulating the polarization of M1 and M2 macrophages.

Liu P, Li H, Gong J, Geng Y, Jiang M, Xu H, Xu Z, Shi J
Food & function 2022 Jan 24;13(2):753-768

One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing.

Guo S, Yao M, Zhang D, He Y, Chang R, Ren Y, Guan F
Advanced healthcare materials 2022 Feb;11(4):e2101808

A Multicenter, Single-Blind, Case-Control, Immunohistochemical Study of Orbital Tissue in Thyroid Eye Disease.

Hai YP, Saeed MEM, Ponto KA, Elflein HM, Lee ACH, Fang S, Zhou H, Frommer L, Längericht J, Efferth T, Kahaly GJ
Thyroid : official journal of the American Thyroid Association 2022 Dec;32(12):1547-1558

Circular RNA circWWC3 augments breast cancer progression through promoting M2 macrophage polarization and tumor immune escape via regulating the expression and secretion of IL-4.

Zheng Y, Ren S, Zhang Y, Liu S, Meng L, Liu F, Gu L, Ai N, Sang M
Cancer cell international 2022 Aug 22;22(1):264

Pentraxin-3 inhibits milky spots metastasis of gastric cancer by inhibiting M2 macrophage polarization.

Cui X, Qin T, Zhao Z, Yang G, Sanches JGP, Zhang Q, Fan S, Cao L, Hu X
Journal of Cancer 2021;12(15):4686-4697

Nuclear factor erythroid 2-related factor 2 potentiates the generation of inflammatory cytokines by intestinal epithelial cells during hyperoxia by inducing the expression of interleukin 17D.

Liu X, Li T, Liu Y, Sun S, Liu D
Toxicology 2021 Jun 15;457:152820

STAT6 up-regulation amplifies M2 macrophage anti-inflammatory capacity through mesenchymal stem cells.

Li Y, Sheng Q, Zhang C, Han C, Bai H, Lai P, Fan Y, Ding Y, Dou X
International immunopharmacology 2021 Feb;91:107266

Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis.

Han Z, Wang H, Guo D, Zhang J
Aging 2021 Feb 1;13(4):5087-5103

DJ-1 Regulates Microglial Polarization Through P62-Mediated TRAF6/IRF5 Signaling in Cerebral Ischemia-Reperfusion.

Wang T, Zhao N, Peng L, Li Y, Huang X, Zhu J, Chen Y, Yu S, Zhao Y
Frontiers in cell and developmental biology 2020;8:593890

Interleukin-4 Restores Insulin Sensitivity in Insulin-Resistant Osteoblasts by Increasing the Expression of Insulin Receptor Substrate 1.

Chao R, Li D, Yue Z, Huang C, Kou Y, Zhou Q, Gao Y, Hasegawa T, Guo J, Li M
Biochemistry. Biokhimiia 2020 Mar;85(3):334-343

Microglia polarization of hippocampus is involved in the mechanism of Apelin-13 ameliorating chronic water immersion restraint stress-induced depression-like behavior in rats.

Zhou S, Chen S, Xie W, Guo X, Zhao J
Neuropeptides 2020 Jun;81:102006

Beneficial Effects of Theta-Burst Transcranial Magnetic Stimulation on Stroke Injury via Improving Neuronal Microenvironment and Mitochondrial Integrity.

Zong X, Dong Y, Li Y, Yang L, Li Y, Yang B, Tucker L, Zhao N, Brann DW, Yan X, Hu S, Zhang Q
Translational stroke research 2020 Jun;11(3):450-467

Neural stem cells induce M2 polarization of macrophages through the upregulation of interleukin-4.

Ji Z, Jiang X, Li Y, Song J, Chai C, Lu X
Experimental and therapeutic medicine 2020 Dec;20(6):148

Comparative Immunopathology of Cetacean morbillivirus Infection in Free-Ranging Dolphins From Western Mediterranean, Northeast-Central, and Southwestern Atlantic.

Díaz-Delgado J, Groch KR, Ressio R, Riskallah IPJ, Sierra E, Sacchini S, Quesada-Canales Ó, Arbelo M, Fernández A, Santos-Neto E, Ikeda J, de Carvalho RR, Azevedo AF, Lailson-Brito J Jr, Flach L, Kanamura CT, Fernandes NCCA, Cogliati B, Centelleghe C, Mazzariol S, Di Renzo L, Di Francesco G, Di Guardo G, Catão-Dias JL
Frontiers in immunology 2019;10:485

Macrophage polarization in human gingival tissue in response to periodontal disease.

Zhou LN, Bi CS, Gao LN, An Y, Chen F, Chen FM
Oral diseases 2019 Jan;25(1):265-273

Juxtaposition of IL-1β and IFN-γ expression and apoptosis of keratinocytes in adult-onset Still's disease.

Rao S, Li Q, Wu H, Zhao M, Wang A, Zhang G, Li J, Lu L, Shi W, Lu Q
Expert review of clinical immunology 2019 Dec;15(12):1341-1350

Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.

Yang L, Tucker D, Dong Y, Wu C, Lu Y, Li Y, Zhang J, Liu TC, Zhang Q
Experimental neurology 2018 Jan;299(Pt A):86-96

Pre-transplant infusion of donor-derived dendritic cells maintained at the immature stage by sinomenine increases splenic Foxp3(+) Tregs in recipient rats after renal allotransplantation.

Li L, Luo Z, Song Z, Zheng L, Chen T
Transplant immunology 2017 Dec;45:22-28

Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.

Wu YH, Liu W, Xue B, Zhang L, Liu XY, Liu B, Wang Y, Cai Y, Duan R
DNA and cell biology 2016 Dec;35(12):853-860

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The IL-4 antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human IL-4. This antibody recognizes human antigen. The IL-4 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 66142-1-Ig.pdf

: Show more

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66142-1-Ig( IL4 Antibody) at dilution of 1:1000
Sample type: cell line