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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAF204 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-4 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-4 in ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
NP_000580- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Decreased allergy incidence in children supplemented with E. coli O83:K24:H31 and its possible modes of action.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Hrdý J, Vlasáková K, Černý V, Súkeníková L, Novotná O, Petrásková P, Boráková K, Lodinová-Žádníková R, Kolářová L, Prokešová L
European journal of immunology 2018 Dec;48(12):2015-2030
European journal of immunology 2018 Dec;48(12):2015-2030
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
Liotta F, Angeli R, Cosmi L, Filì L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
Stem cells (Dayton, Ohio) 2008 Jan;26(1):279-89
Stem cells (Dayton, Ohio) 2008 Jan;26(1):279-89
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Pavón L, Sandoval-López G, Eugenia Hernández M, Loría F, Estrada I, Pérez M, Moreno J, Avila U, Leff P, Antón B, Heinze G
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-4 in Human PBMCs. IL-4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Goat Anti-Human IL-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF204) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
