Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 14714-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14714-1-AP, RRID:AB_2280445
- Product name
- NF45 antibody
- Antibody type
- Polyclonal
- Description
- NF45 antibody (Cat. #14714-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape.
Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing.
Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.
LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex.
Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.
Interleukin-2 enhancer binding factor 2 interacts with the nsp9 or nsp2 of porcine reproductive and respiratory syndrome virus and exerts negatively regulatory effect on the viral replication.
An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.
Cao Y, Wu J, Hu Y, Chai Y, Song J, Duan J, Zhang S, Xu X
Cell reports 2023 Oct 31;42(10):113262
Cell reports 2023 Oct 31;42(10):113262
Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing.
Yan G, Yang J, Li W, Guo A, Guan J, Liu Y
Nature cell biology 2023 May;25(5):754-764
Nature cell biology 2023 May;25(5):754-764
Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.
Zhi S, Congcong Z, Zhiling G, Yihan Q, Yijing X, Guanjie L, Fang W, Xuehua S, Hongjie L, Xiaoni K, Yueqiu G
International journal of biological sciences 2022;18(8):3298-3312
International journal of biological sciences 2022;18(8):3298-3312
LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex.
Li D, Shen L, Zhang X, Chen Z, Huang P, Huang C, Qin S
Journal of experimental & clinical cancer research : CR 2022 Dec 2;41(1):332
Journal of experimental & clinical cancer research : CR 2022 Dec 2;41(1):332
Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.
Ma Y, Liu S, Gao J, Chen C, Zhang X, Yuan H, Chen Z, Yin X, Sun C, Mao Y, Zhou F, Shao Y, Liu Q, Xu J, Cheng L, Yu D, Li P, Yi P, He J, Geng G, Guo Q, Si Y, Zhao H, Li H, Banes GL, Liu H, Nakamura Y, Kurita R, Huang Y, Wang X, Wang F, Fang G, Engel JD, Shi L, Zhang YE, Yu J
Developmental cell 2021 Feb 22;56(4):478-493.e11
Developmental cell 2021 Feb 22;56(4):478-493.e11
Interleukin-2 enhancer binding factor 2 interacts with the nsp9 or nsp2 of porcine reproductive and respiratory syndrome virus and exerts negatively regulatory effect on the viral replication.
Wen X, Bian T, Zhang Z, Zhou L, Ge X, Han J, Guo X, Yang H, Yu K
Virology journal 2017 Jul 11;14(1):125
Virology journal 2017 Jul 11;14(1):125
An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.
Yu Y, Pan X, Ding Y, Liu X, Tang H, Shen C, Shen H, Yang P
The Analyst 2013 Aug 21;138(16):4505-11
The Analyst 2013 Aug 21;138(16):4505-11
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 14714-1-AP(NF45 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using ILF2 antibody 14714-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 14714-1-AP(NF45 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 14714-1-AP(NF45 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue