LS-C806523

antibody from LSBio

Targeting: MCM4 CDC21, CDC54, hCdc21, MGC33310, P1-Cdc21

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C806523

Provider

LSBio

Product name

MCM4 Antibody LS-C806523

Antibody type

Polyclonal

Description

Peptide affinity chromatography.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of HepG2 cell lysates using MCM4 antibody. The lane on the left is treated with the antigen-specific peptide.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of HepG2 cell lysates using MCM4 antibody. The lane on the left is treated with the antigen-specific peptide.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

1:100 staining rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

1:100 staining rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.