NBP1-33105

antibody from Novus Biologicals

Targeting: MCM4 CDC21, CDC54, hCdc21, MGC33310, P1-Cdc21

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-33105

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-33105, RRID:AB_10004031

Product name

Rabbit Polyclonal MCM4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MCM4 Antibody [NBP1-33105] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCM4 antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MCM4 Antibody [NBP1-33105] - MCM4 antibody detects MCM4 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug NCI-H929 whole cell lysate/extract. 7.5 % SDS-PAGE. MCM4 antibody dilution: 1:5000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MCM4 Antibody [NBP1-33105] - (30 ug of whole cell lysate) MOLT4 7. 5% SDS PAGE, antibody diluted at 1:5000.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-33105] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation: MCM4 Antibody [NBP1-33105] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-MCM3 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.