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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-78484 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRD7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HeLa, HeLa nuclear extract
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRD7 in HeLa cells using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with BRD7 polyclonal antibody (Product # PA5-78484) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRD7 antibody detects BRD7 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BRD7 antibody BRD7 Polyclonal Antibody (Product # PA5-78484) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRD7 Polyclonal Antibody detects BRD7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD7 protein stained by BRD7 Polyclonal Antibody (Product # PA5-78484) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
