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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-32188 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLAG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PLAG1-transfected 293T, mouse fetal liver.
- Concentration
- 0.78 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLAG1 using 30 µg of PC-3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PLAG1 polyclonal antibody (Product # PA5-32188) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PLAG1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PLAG1 Polyclonal Antibody (Product # PA5-32188) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PLAG1 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PLAG1 Polyclonal Antibody (Product # PA5-32188) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
