Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [7]
- Other assay [1]
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- Product number
- PA5-64167 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LY6D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ESCTPSYTLQ GQVSSGTSST QCCQEDLCNE KLHNAAP Highest antigen sequence identity to the following orthologs: Mouse - 65%, Rat - 59%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Molecular mapping of interstitial lung disease reveals a phenotypically distinct senescent basal epithelial cell population.
DePianto DJ, Heiden JAV, Morshead KB, Sun KH, Modrusan Z, Teng G, Wolters PJ, Arron JR
JCI insight 2021 Apr 22;6(8)
JCI insight 2021 Apr 22;6(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of LY6D in control (vector only transfected HEK293T lysate) and LY6D over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a LY6D Polyclonal Antibody (Product # PA5-64167).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LY6D in human kidney using LY6D Polyclonal Antibody (Product # PA5-64167).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LY6D in human colon using LY6D Polyclonal Antibody (Product # PA5-64167).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LY6D in human liver using LY6D Polyclonal Antibody (Product # PA5-64167).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LY6D in human esophagus and small intestine tissues using LY6D Polyclonal Antibody (Product # PA5-64167). Corresponding LY6D RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LY6D in human colon, esophagus, kidney and liver using LY6D Polyclonal Antibody (Product # PA5-64167) (A) shows similar protein distribution across tissues to an independent LY6D Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LY6D in human small intestine using LY6D Polyclonal Antibody (Product # PA5-64167) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LY6D in human esophagus using LY6D Polyclonal Antibody (Product # PA5-64167) shows high expression.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Identification of a senescent BEC population enriched in the fibrotic lung. ( A ) Boxplots showing the distribution and mean signature scores for both epithelial cell-derived and fibroblast-derived consensus senescence gene sets; each point reflects the mean signature score for an individual subject within the given cluster. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. ( B ) Heatmap showing the log fold change in normalized expression between the Basal-1 and Basal-2 populations for genes in the union set of control, IPF, and SSc-ILD differentially expressed genes across these 2 clusters; genes upregulated in Basal-2 are shown in red, and genes upregulated in Basal-1 are shown in blue. ( C ) IHC staining of control, IPF, and SSc-ILD lung tissue sections for LY6D. ( D ) IHC staining of IPF lung tissue serial sections for LY6D and p16. ( E ) Scoring of clusters E1-6, from Figure 4 , against Basal1/2 gene expression signatures. Scale bars: 100 mum ( C ), 40 mum ( D ).