Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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- Product number
- PA5-85603 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC61B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution. Predicted reactivity: Dog (100%), Pig (100%). Positive Control: 293T, A431, HeLa, HepG2, mouse liver. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.77 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEC61B in mouse tissue extracts using SEC61B polyclonal antibody (Product # PA5-85603) using 50 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEC61B in various whole cell extracts using SEC61B polyclonal antibody (Product # PA5-85603) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEC61B Polyclonal Antibody detects SEC61B protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 15 % SDS-PAGE, and the membrane was blotted with SEC61B Polyclonal Antibody (Product # PA5-85603) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEC61B Polyclonal Antibody detects SEC61B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SEC61B Polyclonal Antibody (Product # PA5-85603) diluted at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of SEC61B in HepG2 whole cell extracts with SEC61B polyclonal antibody (Product # PA5-85603) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.