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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-71911 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-71911, RRID:AB_11027614
- Product name
- Rabbit Polyclonal CBF2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: CBF2 Antibody [NBP1-71911] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-C/EBP zeta antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. C/EBP zeta was also immunoprecipitated by rabbit anti-C/EBP zeta antibodies NBP1-71908, NBP1-71909 and NBP1-71910, which recognize upstream epitopes.
