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- References [18]
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- Product number
- 14662-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14662-1-AP, RRID:AB_2242609
- Product name
- AFF4 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated AFF4 antibody (Cat. #14662-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Discovery of a First-in-Class Murine Double Minute 2-Recruiting Positive Transcription Elongation Factor B PROTAC Degrader With Selective Antitumor Activity.
MeCP2 interacts with the super elongation complex to regulate transcription.
Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal.
N6-methyladenosine-modified circCDK14 promotes ossification of the ligamentum flavum via epigenetic modulation by targeting AFF4.
Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal.
MeCP2 Interacts with the Super Elongation Complex to Regulate Transcription.
AFF4 regulates osteogenic potential of human periodontal ligament stem cells via mTOR-ULK1-autophagy axis.
Inherited blood cancer predisposition through altered transcription elongation.
Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription.
CSTF2 mediated mRNA N(6)-methyladenosine modification drives pancreatic ductal adenocarcinoma m(6)A subtypes.
TMT proteomics analysis reveals the mechanism of bleomycin-induced pulmonary fibrosis and effects of Ginseng honeysuckle superfine powdered tea.
RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.
Transcription Elongation Machinery Is a Druggable Dependency and Potentiates Immunotherapy in Glioblastoma Stem Cells.
Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.
Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription.
Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.
A chalcone derivative reactivates latent HIV-1 transcription through activating P-TEFb and promoting Tat-SEC interaction on viral promoter.
Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
Guan X, Xie L, Guo H, Ma L, Zhou J, Luo L, Yang H, Wu Y, Liu J, Wang Y, Huang X, Liu J, Zhang Y, Chen W, Chen Y, Xu L, Han X
MedComm 2026 Apr;7:e70723
MedComm 2026 Apr;7:e70723
MeCP2 interacts with the super elongation complex to regulate transcription.
Sonn JY, Kim W, Iwanaszko M, Aoi Y, Li Y, Qi G, Parkitny L, Brissette JL, Weiner L, Botas J, Al-Ramahi I, Shilatifard A, Zoghbi HY
Science advances 2025 Nov 28;11(48):eadt5937
Science advances 2025 Nov 28;11(48):eadt5937
Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal.
Cisneros WJ, Soliman SHA, Walter M, Simons LM, Cornish D, De Fabritiis S, Halle AW, Kim EY, Wolinsky SM, Lorenzo-Redondo R, Shilatifard A, Hultquist JF
PLoS pathogens 2024 Sep;20(9):e1012083
PLoS pathogens 2024 Sep;20(9):e1012083
N6-methyladenosine-modified circCDK14 promotes ossification of the ligamentum flavum via epigenetic modulation by targeting AFF4.
Zhao Y, Chen L, Xiang Q, Lin J, Jiang S, Li W
Cellular and molecular life sciences : CMLS 2024 Oct 16;81(1):436
Cellular and molecular life sciences : CMLS 2024 Oct 16;81(1):436
Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal.
Cisneros WJ, Walter M, Soliman SHA, Simons LM, Cornish D, Halle AW, Kim EY, Wolinsky SM, Shilatifard A, Hultquist JF
bioRxiv : the preprint server for biology 2024 Mar 1;
bioRxiv : the preprint server for biology 2024 Mar 1;
MeCP2 Interacts with the Super Elongation Complex to Regulate Transcription.
Sonn JY, Kim W, Iwanaszko M, Aoi Y, Li Y, Parkitny L, Brissette JL, Weiner L, Al-Ramahi I, Botas J, Shilatifard A, Zoghbi HY
bioRxiv : the preprint server for biology 2024 Jul 1;
bioRxiv : the preprint server for biology 2024 Jul 1;
AFF4 regulates osteogenic potential of human periodontal ligament stem cells via mTOR-ULK1-autophagy axis.
Zhu L, Wang J, Wu Z, Chen S, He Y, Jiang Y, Luo G, Wu Z, Li Y, Xie J, Zou S, Zhou C
Cell proliferation 2024 Feb;57(2):e13546
Cell proliferation 2024 Feb;57(2):e13546
Inherited blood cancer predisposition through altered transcription elongation.
Zhao J, Cato LD, Arora UP, Bao EL, Bryant SC, Williams N, Jia Y, Goldman SR, Nangalia J, Erb MA, Vos SM, Armstrong SA, Sankaran VG
Cell 2024 Feb 1;187(3):642-658.e19
Cell 2024 Feb 1;187(3):642-658.e19
Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription.
Lau MS, Hu Z, Zhao X, Tan YS, Liu J, Huang H, Yeo CJ, Leong HF, Grinchuk OV, Chan JK, Yan J, Tee WW
Nature communications 2023 Oct 13;14(1):6464
Nature communications 2023 Oct 13;14(1):6464
CSTF2 mediated mRNA N(6)-methyladenosine modification drives pancreatic ductal adenocarcinoma m(6)A subtypes.
Zheng Y, Li X, Deng S, Zhao H, Ye Y, Zhang S, Huang X, Bai R, Zhuang L, Zhou Q, Li M, Su J, Li R, Bao X, Zeng L, Chen R, Zheng J, Lin D, He C, Zhang J, Zuo Z
Nature communications 2023 Oct 10;14(1):6334
Nature communications 2023 Oct 10;14(1):6334
TMT proteomics analysis reveals the mechanism of bleomycin-induced pulmonary fibrosis and effects of Ginseng honeysuckle superfine powdered tea.
Li X, Yu X, Gao Y, Zhao W, Wang Y, Yu F, Fu C, Gao H, Cheng M, Li B
Chinese medicine 2023 May 24;18(1):60
Chinese medicine 2023 May 24;18(1):60
RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits.
Li Y, Huang J, Bao L, Zhu J, Duan W, Zheng H, Wang H, Jiang Y, Liu W, Zhang M, Yu Y, Yi C, Ji X
Molecular cell 2023 Apr 20;83(8):1280-1297.e11
Molecular cell 2023 Apr 20;83(8):1280-1297.e11
Transcription Elongation Machinery Is a Druggable Dependency and Potentiates Immunotherapy in Glioblastoma Stem Cells.
Qiu Z, Zhao L, Shen JZ, Liang Z, Wu Q, Yang K, Min L, Gimple RC, Yang Q, Bhargava S, Jin C, Kim C, Hinz D, Dixit D, Bernatchez JA, Prager BC, Zhang G, Dong Z, Lv D, Wang X, Kim LJY, Zhu Z, Jones KA, Zheng Y, Wang X, Siqueira-Neto JL, Chavez L, Fu XD, Spruck C, Rich JN
Cancer discovery 2022 Feb;12(2):502-521
Cancer discovery 2022 Feb;12(2):502-521
Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.
Zheng B, Aoi Y, Shah AP, Iwanaszko M, Das S, Rendleman EJ, Zha D, Khan N, Smith ER, Shilatifard A
Genes & development 2021 Feb 1;35(3-4):273-285
Genes & development 2021 Feb 1;35(3-4):273-285
Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription.
Tang D, Chen C, Liao G, Liu J, Liao B, Huang Q, Chen Q, Zhao J, Jiang H, Duan J, Huang J, Wang K, Wang J, Zhou C, Chu W, Li W, Sun B, Li Z, Dai L, Fu X, Cheng W, Xue Y, Qi S
Cell discovery 2020;6:7
Cell discovery 2020;6:7
Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.
Liang K, Smith ER, Aoi Y, Stoltz KL, Katagi H, Woodfin AR, Rendleman EJ, Marshall SA, Murray DC, Wang L, Ozark PA, Mishra RK, Hashizume R, Schiltz GE, Shilatifard A
Cell 2018 Oct 18;175(3):766-779.e17
Cell 2018 Oct 18;175(3):766-779.e17
A chalcone derivative reactivates latent HIV-1 transcription through activating P-TEFb and promoting Tat-SEC interaction on viral promoter.
Wu J, Ao MT, Shao R, Wang HR, Yu D, Fang MJ, Gao X, Wu Z, Zhou Q, Xue YH
Scientific reports 2017 Sep 6;7(1):10657
Scientific reports 2017 Sep 6;7(1):10657
Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
Lu X, Zhu X, Li Y, Liu M, Yu B, Wang Y, Rao M, Yang H, Zhou K, Wang Y, Chen Y, Chen M, Zhuang S, Chen LF, Liu R, Chen R
Nucleic acids research 2016 Aug 19;44(14):6853-67
Nucleic acids research 2016 Aug 19;44(14):6853-67
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The AFF4 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human AFF4. This antibody recognizes human, mouse, rat antigen. The AFF4 antibody has been validated for the following applications: ELISA, WB analysis.
