Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00027125-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00027125-M01, RRID:AB_536637
- Product name
- Mouse Monoclonal AFF4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AFF4 - AF4/FMR2 family, member 4
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AFF4 Antibody (2E12) [H00027125-M01] - Analysis of AFF4 expression in transfected 293T cell line by AFF4 monoclonal antibody (M01), clone 2E12.Lane 1: AFF4 transfected lysate( 40.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: AFF4 Antibody (2E12) [H00027125-M01] - Detection limit for recombinant GST tagged AFF4 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AFF4 Antibody (2E12) [H00027125-M01] - Analysis of monoclonal antibody to AFF4 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 1 ug/ml.
