Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP2-29674 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal PRKD3/nPKC nu Antibody
- Antibody type
- Monoclonal
- Description
- Tissue culture supernatant. phospho-PKD 105-110kDa weight may be detected.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protein Kinase D3 [p Ser916] Antibody (MC29) [NBP2-29674] - Unstimulated (lanes 1 and 3) or PDBu-stimulated(lanes 2 and 4) HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKD (1:2000, lanes 1 and 2) or anti-PKD . Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKD (110 kDa).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protein Kinase D3 [p Ser916] Antibody (MC29) [NBP2-29674] - Unstimulated (lane 2) or PDBu-stimulated (lane 1) HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKD. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKD (110 kDa).