NBP2-29674

antibody from Novus Biologicals

Targeting: PRKD3 EPK2, PRKCN

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-29674

Provider

Novus Biologicals

Product name

Rabbit Monoclonal PRKD3/nPKC nu Antibody

Antibody type

Monoclonal

Description

Tissue culture supernatant. phospho-PKD 105-110kDa weight may be detected.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Protein Kinase D3 [p Ser916] Antibody (MC29) [NBP2-29674] - Unstimulated (lanes 1 and 3) or PDBu-stimulated(lanes 2 and 4) HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKD (1:2000, lanes 1 and 2) or anti-PKD . Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKD (110 kDa).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Protein Kinase D3 [p Ser916] Antibody (MC29) [NBP2-29674] - Unstimulated (lane 2) or PDBu-stimulated (lane 1) HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKD. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKD (110 kDa).