Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-2316 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2316, RRID:AB_10000964
- Product name
- Rabbit Polyclonal PRKD3/nPKC nu Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Protein kinase D regulates cofilin activity through p21-activated kinase 4.
Protein kinase D2 is a crucial regulator of tumour cell-endothelial cell communication in gastrointestinal tumours.
Lysophosphatidylcholine activates a novel PKD2-mediated signaling pathway that controls monocyte migration.
PKD3 is the predominant protein kinase D isoform in mouse exocrine pancreas and promotes hormone-induced amylase secretion.
Vascular endothelial growth factor induces protein kinase D-dependent production of proinflammatory cytokines in endothelial cells.
Phosphorylation of the Par-1 polarity kinase by protein kinase D regulates 14-3-3 binding and membrane association.
Spratley SJ, Bastea LI, Döppler H, Mizuno K, Storz P
The Journal of biological chemistry 2011 Sep 30;286(39):34254-61
The Journal of biological chemistry 2011 Sep 30;286(39):34254-61
Protein kinase D2 is a crucial regulator of tumour cell-endothelial cell communication in gastrointestinal tumours.
Azoitei N, Pusapati GV, Kleger A, Möller P, Küfer R, Genze F, Wagner M, van Lint J, Carmeliet P, Adler G, Seufferlein T
Gut 2010 Oct;59(10):1316-30
Gut 2010 Oct;59(10):1316-30
Lysophosphatidylcholine activates a novel PKD2-mediated signaling pathway that controls monocyte migration.
Tan M, Hao F, Xu X, Chisolm GM, Cui MZ
Arteriosclerosis, thrombosis, and vascular biology 2009 Sep;29(9):1376-82
Arteriosclerosis, thrombosis, and vascular biology 2009 Sep;29(9):1376-82
PKD3 is the predominant protein kinase D isoform in mouse exocrine pancreas and promotes hormone-induced amylase secretion.
Chen LA, Li J, Silva SR, Jackson LN, Zhou Y, Watanabe H, Ives KL, Hellmich MR, Evers BM
The Journal of biological chemistry 2009 Jan 23;284(4):2459-71
The Journal of biological chemistry 2009 Jan 23;284(4):2459-71
Vascular endothelial growth factor induces protein kinase D-dependent production of proinflammatory cytokines in endothelial cells.
Hao Q, Wang L, Tang H
American journal of physiology. Cell physiology 2009 Apr;296(4):C821-7
American journal of physiology. Cell physiology 2009 Apr;296(4):C821-7
Phosphorylation of the Par-1 polarity kinase by protein kinase D regulates 14-3-3 binding and membrane association.
Watkins JL, Lewandowski KT, Meek SE, Storz P, Toker A, Piwnica-Worms H
Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 25;105(47):18378-83
Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 25;105(47):18378-83
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protein Kinase D3 Antibody [NB100-2316] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PKD3 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).