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ImmunohistochemistryAntibody data
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- Antigen structure
- References [17]
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- Validations
- Immunocytochemistry [1]
- Other assay [4]
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- Product number
- 35-4900 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MUC4 Monoclonal Antibody (1G8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1G8
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Isolation and characterization of adult mammary stem cells from breast cancer-adjacent tissues.
Effects of acute intra-abdominal hypertension on multiple intestinal barrier functions in rats.
Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.
Tau and PTEN status as predictive markers for response to trastuzumab and paclitaxel in patients with HER2-positive breast cancer.
Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.
Universal antibody conjugation to nanoparticles using the Fcγ receptor I (FcγRI): quantitative profiling of membrane biomarkers.
In situ drug-receptor binding kinetics in single cells: a quantitative label-free study of anti-tumor drug resistance.
Pancreatic cancer counterattack: MUC4 mediates Fas-independent apoptosis of antigen-specific cytotoxic T lymphocyte.
Prospective randomized controlled study comparing cell block method and conventional smear method for bile cytology.
Transmembrane mucin MUC1 overexpression and its association with CD10⁺ myeloid cells, transforming growth factor-β1 expression, and tumor budding grade in colorectal cancer.
Mucin 16 is a functional selectin ligand on pancreatic cancer cells.
Characterization of a novel rat cholangiocarcinoma cell culture model-CGCCA.
AMPK induces MUC5B expression via p38 MAPK in NCI-H292 airway epithelial cells.
Leptin up-regulates MUC5B expression in human airway epithelial cells via mitogen-activated protein kinase pathway.
Prognostic value of MUC4 for mass-forming intrahepatic cholangiocarcinoma after hepatectomy.
Glycoproteomics of milk: differences in sugar epitopes on human and bovine milk fat globule membranes.
Differential regulation of membrane-associated mucins in the human ocular surface epithelium.
Shi AP, Fan ZM, Ma KW, Jiang YF, Wang L, Zhang KW, Fu SB, Xu N, Zhang ZR
Oncology letters 2017 Sep;14(3):2894-2902
Oncology letters 2017 Sep;14(3):2894-2902
Effects of acute intra-abdominal hypertension on multiple intestinal barrier functions in rats.
Leng Y, Yi M, Fan J, Bai Y, Ge Q, Yao G
Scientific reports 2016 Mar 16;6:22814
Scientific reports 2016 Mar 16;6:22814
Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.
Ertoy Baydar D, Kosemehmetoglu K, Aydin O, Bridge JA, Buyukeren B, Aki FT
Diagnostic pathology 2015 Oct 9;10:186
Diagnostic pathology 2015 Oct 9;10:186
Tau and PTEN status as predictive markers for response to trastuzumab and paclitaxel in patients with HER2-positive breast cancer.
Koo DH, Lee HJ, Ahn JH, Yoon DH, Kim SB, Gong G, Son BH, Ahn SH, Jung KH
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Aug;36(8):5865-71
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Aug;36(8):5865-71
Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.
Dhir A, Dhir S, Proudfoot NJ, Jopling CL
Nature structural & molecular biology 2015 Apr;22(4):319-27
Nature structural & molecular biology 2015 Apr;22(4):319-27
Universal antibody conjugation to nanoparticles using the Fcγ receptor I (FcγRI): quantitative profiling of membrane biomarkers.
Kim C, Galloway JF, Lee KH, Searson PC
Bioconjugate chemistry 2014 Oct 15;25(10):1893-901
Bioconjugate chemistry 2014 Oct 15;25(10):1893-901
In situ drug-receptor binding kinetics in single cells: a quantitative label-free study of anti-tumor drug resistance.
Wang W, Yin L, Gonzalez-Malerva L, Wang S, Yu X, Eaton S, Zhang S, Chen HY, LaBaer J, Tao N
Scientific reports 2014 Oct 14;4:6609
Scientific reports 2014 Oct 14;4:6609
Pancreatic cancer counterattack: MUC4 mediates Fas-independent apoptosis of antigen-specific cytotoxic T lymphocyte.
Zhu Y, Zhang JJ, Liang WB, Zhu R, Wang B, Miao Y, Xu ZK
Oncology reports 2014 Apr;31(4):1768-76
Oncology reports 2014 Apr;31(4):1768-76
Prospective randomized controlled study comparing cell block method and conventional smear method for bile cytology.
Noda Y, Fujita N, Kobayashi G, Ito K, Horaguchi J, Hashimoto S, Koshita S, Ishii S, Kanno Y, Ogawa T, Masu K, Tsuchiya T, Oikawa M, Honda H, Sawai T, Uzuki M, Fujishima F
Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society 2013 Jul;25(4):444-52
Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society 2013 Jul;25(4):444-52
Transmembrane mucin MUC1 overexpression and its association with CD10⁺ myeloid cells, transforming growth factor-β1 expression, and tumor budding grade in colorectal cancer.
Khanh do T, Mekata E, Mukaisho K, Sugihara H, Shimizu T, Shiomi H, Murata S, Naka S, Yamamoto H, Endo Y, Tani T
Cancer science 2013 Jul;104(7):958-64
Cancer science 2013 Jul;104(7):958-64
Mucin 16 is a functional selectin ligand on pancreatic cancer cells.
Chen SH, Dallas MR, Balzer EM, Konstantopoulos K
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Mar;26(3):1349-59
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Mar;26(3):1349-59
Characterization of a novel rat cholangiocarcinoma cell culture model-CGCCA.
Yeh CN, Lin KJ, Chen TW, Wu RC, Tsao LC, Chen YT, Weng WH, Chen MF
World journal of gastroenterology 2011 Jun 28;17(24):2924-32
World journal of gastroenterology 2011 Jun 28;17(24):2924-32
AMPK induces MUC5B expression via p38 MAPK in NCI-H292 airway epithelial cells.
Bae CH, Kim JW, Ye SB, Song SY, Kim YW, Park SY, Kim YD
Biochemical and biophysical research communications 2011 Jun 17;409(4):669-74
Biochemical and biophysical research communications 2011 Jun 17;409(4):669-74
Leptin up-regulates MUC5B expression in human airway epithelial cells via mitogen-activated protein kinase pathway.
Woo HJ, Yoo WJ, Bae CH, Song SY, Kim YW, Park SY, Kim YD
Experimental lung research 2010 Jun;36(5):262-9
Experimental lung research 2010 Jun;36(5):262-9
Prognostic value of MUC4 for mass-forming intrahepatic cholangiocarcinoma after hepatectomy.
Yeh CN, Pang ST, Wu RC, Chen TW, Jan YY, Chen MF
Oncology reports 2009 Jan;21(1):49-56
Oncology reports 2009 Jan;21(1):49-56
Glycoproteomics of milk: differences in sugar epitopes on human and bovine milk fat globule membranes.
Wilson NL, Robinson LJ, Donnet A, Bovetto L, Packer NH, Karlsson NG
Journal of proteome research 2008 Sep;7(9):3687-96
Journal of proteome research 2008 Sep;7(9):3687-96
Differential regulation of membrane-associated mucins in the human ocular surface epithelium.
Hori Y, Spurr-Michaud S, Russo CL, Argüeso P, Gipson IK
Investigative ophthalmology & visual science 2004 Jan;45(1):114-22
Investigative ophthalmology & visual science 2004 Jan;45(1):114-22
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MUC4/Mucin 4 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUC4/Mucin 4 (1G8) Mouse Monoclonal Antibody (Product # 35-4900) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 12 Case #2. SMA stains interspersed myofibroblasts and MUC4 labels neoplastic cells ( Left : Immunohistochemistry, anti-SMA Ab x 200; Right : Immunohistochemistry, anti-MUC4 Ab x 200)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Case #1. SMA stains myofibroblasts between neoplastic lobules and MUC4 stains neoplastic cells ( Left : Immunohistochemistry, anti-SMA Ab x 100; Right : Immunohistochemistry, anti-MUC4 Ab x 400)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Quantitativeexpression levels for CLDN4, MSLN, MUC4, and CDH11on Panc-1, MIA PaCa-2, Capan-1, and HPDE cells. (A) phase and fluorescenceimages of MiaPaCa-2, Panc-1, Capan-1, and HPDE cells incubated withQD-FcR-Ab conjugates (Ab = aCLDN4, aMSLN, aMUC4, and aCDH11). Biomarkerexpression levels on (B) MIA PaCa-2, (C) Panc-1, (D) Capan-1, and(E) HPDE cells. Error bars represent standard error. Expression levelsfor each biomarker on MIA PaCa-2, Panc-1, and HPDE are obtained frommeasurements of between 565-997 individual cells (see Supporting Information Figure S2 for details).For Capan-1, expression levels are based on measurements of at least180 clusters of cells. In total, measurements were made on more than9600 individual cells and more than 780 cell clusters.
