V7542

antibody from NSJ Bioreagents

Targeting: MUC4

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V7542

Provider

NSJ Bioreagents

Product name

MUC4 Antibody / Mucin-4

Antibody type

Monoclonal

Description

Protein G affinity chromatography

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)

Storage

Store the MUC4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human gastric carcinoma with MUC4 antibody (clone MUC4/3105). HIER: requires steaming of sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon carcinoma with MUC4 antibody (clone MUC4/3105). HIER: requires steaming of sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon carcinoma with MUC4 antibody (clone MUC4/3105). HIER: requires steaming of sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool before testing.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MUC4 antibody (clone MUC4/3105). These results demonstrate the foremost specificity of the MUC4/3105 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free MUC4 antibody (clone MUC4/3105) as confirmation of integrity and purity.