AF1835

antibody from R&D Systems

Targeting: SEMA7A CD108, H-Sema-L, SEMAL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1835

Provider

R&D Systems

Product name

Mouse Semaphorin 7A Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Semaphorin 7A in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, rmSemaphorin 3F, rmSemaphorin 6A, rmSemaphorin 6B, rmSemaphorin 6C, recombinant human (rh) Semaphorin 3A, rhSemaphorin 3B, and rhSemaphorin 6D is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9QUR8


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation.

Inoue N, Nishizumi H, Naritsuka H, Kiyonari H, Sakano H
Nature communications 2018 May 9;9(1):1842

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Rewiring the taste system.

Lee H, Macpherson LJ, Parada CA, Zuker CS, Ryba NJP
Nature 2017 Aug 17;548(7667):330-333

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Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence.

Parkash J, Messina A, Langlet F, Cimino I, Loyens A, Mazur D, Gallet S, Balland E, Malone SA, Pralong F, Cagnoni G, Schellino R, De Marchis S, Mazzone M, Pasterkamp RJ, Tamagnone L, Prevot V, Giacobini P
Nature communications 2015 Feb 27;6:6385

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse Semaphorin 7A by Western Blot. Western blot shows lysates of mouse brain tissue, mouse testis tissue, and mouse ovary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1835) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Semaphorin 7A at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Semaphorin 7A by Simple Western<SUP abp="261">TM. Simple Western lane view shows lysates of human brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 7A at approximately 109 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1835) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.