LS-C18837

antibody from LSBio

Targeting: LIMK1 LIMK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

LS-C18837

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C18837, RRID:AB_2136956

Product name

LIMK1 / LIMK Antibody (aa620-647) LS-C18837

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-LIM Kinase Antibody - Western Blot. Western blot of Affinity Purified anti-LIM Kinase antibody shows detection of a 73 kD band corresponding to LIM kinase in lysates from mouse brain. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LIM kinase antibody. The doublet band at ~75 kD may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 kD is unknown. Signal was detected using standard techniques.