Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-37629 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-LIMK1 (Thr508) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is C2C12, Hela or 293 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Semaphorin3F Drives Dendritic Spine Pruning Through Rho-GTPase Signaling.
Duncan BW, Mohan V, Wade SD, Truong Y, Kampov-Polevoi A, Temple BR, Maness PF
Molecular neurobiology 2021 Aug;58(8):3817-3834
Molecular neurobiology 2021 Aug;58(8):3817-3834
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from UV-treated C2C12, PMA-treated Hela and anisomycin-treated 293 cells using LIMK1 (pThr508) polyclonal antibody (Product # PA5-37629).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of methanol-fixed Hela cells using LIMK1 (pThr508) polyclonal antibody (Product # PA5-37629).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of methanol-fixed Hela cells using LIMK1 (pThr508) polyclonal antibody (Product # PA5-37629).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-LIMK1 (Thr508) in methanol-fixed Hela cells, using Phospho-LIMK1 (Thr508) Polyclonal Antibody (Product # PA5-37629).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-LIMK1 (Thr508) in Lane 1: Hela cells treated with PMA, Lane 2: mouse brain tissue, Lane 3: rat brain tissue, Lane 4: N-peptide blocked Hela cells treated with PMA, Lane 5: N-peptide blocked mouse brain tissue, Lane 6: N-peptide blocked rat brain tissue, Lane 7: P-peptide blocked Hela cells treated with PMA, Lane 8: P-peptide blocked mouse brain tissue, Lane 9: P-peptide blocked Rat brain tissue lysates at 40 µg per lane. Samples were probed using Phospho-LIMK1 (Thr508) Polyclonal Antibody (Product # PA5-37629).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-LIMK1 (Thr508) in paraffin-embedded Human breast carcinoma tissue using Phospho-LIMK1 (Thr508) Polyclonal Antibody (Product # PA5-37629) (left) or the same antibody preincubated with blocking peptide (right).