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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3484 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3484, RRID:AB_1576813
- Product name
- LIMK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of LIMK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop.
Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase.
Structural features of LIM kinase that control effects on the actin cytoskeleton.
Ohashi K, Nagata K, Maekawa M, Ishizaki T, Narumiya S, Mizuno K
The Journal of biological chemistry 2000 Feb 4;275(5):3577-82
The Journal of biological chemistry 2000 Feb 4;275(5):3577-82
Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase.
Maekawa M, Ishizaki T, Boku S, Watanabe N, Fujita A, Iwamatsu A, Obinata T, Ohashi K, Mizuno K, Narumiya S
Science (New York, N.Y.) 1999 Aug 6;285(5429):895-8
Science (New York, N.Y.) 1999 Aug 6;285(5429):895-8
Structural features of LIM kinase that control effects on the actin cytoskeleton.
Edwards DC, Gill GN
The Journal of biological chemistry 1999 Apr 16;274(16):11352-61
The Journal of biological chemistry 1999 Apr 16;274(16):11352-61
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The LIMK1 polyclonal antibody (Cat # PAB3484) is used in Western blot to detect LIMK1 in A-375 cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with LIMK1 polyclonal antibody (Cat # PAB3484) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
