Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB10258 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10258, RRID:AB_1676506
- Product name
- LIMK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of LIMK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references LATS1 tumour suppressor affects cytokinesis by inhibiting LIMK1.
LIM kinase 1, a key regulator of actin dynamics, is widely expressed in embryonic and adult tissues.
p57Kip2 regulates actin dynamics by binding and translocating LIM-kinase 1 to the nucleus.
Yang X, Yu K, Hao Y, Li DM, Stewart R, Insogna KL, Xu T
Nature cell biology 2004 Jul;6(7):609-17
Nature cell biology 2004 Jul;6(7):609-17
LIM kinase 1, a key regulator of actin dynamics, is widely expressed in embryonic and adult tissues.
Foletta VC, Moussi N, Sarmiere PD, Bamburg JR, Bernard O
Experimental cell research 2004 Apr 1;294(2):392-405
Experimental cell research 2004 Apr 1;294(2):392-405
p57Kip2 regulates actin dynamics by binding and translocating LIM-kinase 1 to the nucleus.
Yokoo T, Toyoshima H, Miura M, Wang Y, Iida KT, Suzuki H, Sone H, Shimano H, Gotoda T, Nishimori S, Tanaka K, Yamada N
The Journal of biological chemistry 2003 Dec 26;278(52):52919-23
The Journal of biological chemistry 2003 Dec 26;278(52):52919-23
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using LIMK1 polyclonal antibody (Cat # PAB10258) shows detection of a 73 KDa band corresponding to LIMK1 in lysates from mouse brain.Approximately 18 ug of lysate wasrun on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of LIMK1 polyclonal antibody.The doublet band at ~ 75 KDa may represent phosphorylated and non-phosphorylated forms of the protein.The identity of the strong lower molecular weight band at approximately 20 KDa is unknown.Signal was detected using standard techniques.
