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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA502904 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502904, RRID:AB_11126577
- Product name
- LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone OTI3H6 (formerly 3H6)
- Antibody type
- Monoclonal
- Description
- LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone OTI3H6 (formerly 3H6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LIMK1
- Isotype
- IgG
- Antibody clone number
- OTI3H6
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Transient inhibition of LIMKs significantly attenuated central sensitization and delayed the development of chronic pain.
Yang X, He G, Zhang X, Chen L, Kong Y, Xie W, Jia Z, Liu WT, Zhou Z
Neuropharmacology 2017 Oct;125:284-294
Neuropharmacology 2017 Oct;125:284-294
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (RC218058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIMK1 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-LIMK1 mouse monoclonal antibody (TA502904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1(RC218058).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502904)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502904)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC218058 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIMK1 antibody(TA502904), and then analyzed by flow cytometry.
- Validation comment
- FC
