Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA502977 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502977, RRID:AB_11127768
- Product name
- LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone OTI8B2 (formerly 8B2)
- Antibody type
- Monoclonal
- Description
- LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone OTI8B2 (formerly 8B2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LIMK1
- Isotype
- IgG
- Antibody clone number
- OTI8B2
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (RC218058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-LIMK1 mouse monoclonal antibody (TA502977) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1(RC218058).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC218058 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIMK1 antibody(TA502977), and then analyzed by flow cytometry.
- Validation comment
- FC
