ABIN362107

antibody from antibodies-online

Targeting: LIMK1 LIMK

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362107

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN362107, RRID:AB_10953755

Product name

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pThr508 (R-Y-T (p) -V-V) derived from Human LIMK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

pThr508

Isotype

IgG

Vial size

50 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop.

Ohashi K, Nagata K, Maekawa M, Ishizaki T, Narumiya S, Mizuno K
The Journal of biological chemistry 2000 Feb 4;275(5):3577-82

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Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics.

Edwards DC, Sanders LC, Bokoch GM, Gill GN
Nature cell biology 1999 Sep;1(5):253-9