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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52485PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52485PU-N, RRID:AB_11147978
- Product name
- anti LIMK1 (Thr508)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 493-522 amino acids from Human LIMK1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of LIMK1 (Thr508) antibody Cat.-No AP52485PU-N in K562 cell line lysates (35ug/lane). This demonstrates the LIMK1 antibody detected the LIMK1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LIMK1 (Thr508) antibody Cat.-No AP52485PU-N in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K562 cells using LIMK1 (Thr508) antibody Cat.-No AP52485PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
