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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- NB100-2177 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2177, RRID:AB_10000916
- Product name
- Mouse Monoclonal ABCG2/CD338 Antibody
- Antibody type
- Monoclonal
- Description
- Tissue culture supernatant. This recognizes an internal epitope of human BCRP (70 kDa), a transmembrane half-transporter. The does not cross-react with the human MDR1, MRP1, and MRP2 gene products.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.25 ml
- Concentration
- 0.25 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Alcohol and Cocaine Exposure Modulates ABCB1 and ABCG2 Transporters in Male Alcohol-Preferring Rats.
Cariprazine, A Dopamine D₂/D₃ Receptor Partial Agonist, Modulates ABCG2-Mediated Multidrug Resistance in Cancer.
The dopamine D3 receptor antagonists PG01037, NGB2904, SB277011A, and U99194 reverse ABCG2 transporter-mediated drug resistance in cancer cell lines.
Lysophosphatidate signaling stabilizes Nrf2 and increases the expression of genes involved in drug resistance and oxidative stress responses: implications for cancer treatment.
ABCG2 localizes to the nucleus and modulates CDH1 expression in lung cancer cells.
ABCB1 and ABCG2 proteins, their functional activity and gene expression in concert with drug sensitivity of leukemia cells.
The nature of amino acid 482 of human ABCG2 affects substrate transport and ATP hydrolysis but not substrate binding.
Hammad AM, Alasmari F, Sari Y, Scott Hall F, Tiwari AK
Molecular neurobiology 2019 Mar;56(3):1921-1932
Molecular neurobiology 2019 Mar;56(3):1921-1932
Cariprazine, A Dopamine D₂/D₃ Receptor Partial Agonist, Modulates ABCG2-Mediated Multidrug Resistance in Cancer.
Hussein N, Ashby CR Jr, Amawi H, Nyinawabera A, Vij A, Khare VM, Karthikeyan C, Tiwari AK
Cancers 2018 Sep 4;10(9)
Cancers 2018 Sep 4;10(9)
The dopamine D3 receptor antagonists PG01037, NGB2904, SB277011A, and U99194 reverse ABCG2 transporter-mediated drug resistance in cancer cell lines.
Hussein N, Amawi H, Karthikeyan C, Hall FS, Mittal R, Trivedi P, Ashby CR Jr, Tiwari AK
Cancer letters 2017 Jun 28;396:167-180
Cancer letters 2017 Jun 28;396:167-180
Lysophosphatidate signaling stabilizes Nrf2 and increases the expression of genes involved in drug resistance and oxidative stress responses: implications for cancer treatment.
Venkatraman G, Benesch MG, Tang X, Dewald J, McMullen TP, Brindley DN
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Mar;29(3):772-85
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Mar;29(3):772-85
ABCG2 localizes to the nucleus and modulates CDH1 expression in lung cancer cells.
Liang SC, Yang CY, Tseng JY, Wang HL, Tung CY, Liu HW, Chen CY, Yeh YC, Chou TY, Yang MH, Whang-Peng J, Lin CH
Neoplasia (New York, N.Y.) 2015 Mar;17(3):265-78
Neoplasia (New York, N.Y.) 2015 Mar;17(3):265-78
ABCB1 and ABCG2 proteins, their functional activity and gene expression in concert with drug sensitivity of leukemia cells.
Svirnovski AI, Shman TV, Serhiyenka TF, Savitski VP, Smolnikova VV, Fedasenka UU
Hematology (Amsterdam, Netherlands) 2009 Aug;14(4):204-12
Hematology (Amsterdam, Netherlands) 2009 Aug;14(4):204-12
The nature of amino acid 482 of human ABCG2 affects substrate transport and ATP hydrolysis but not substrate binding.
Ejendal KF, Diop NK, Schweiger LC, Hrycyna CA
Protein science : a publication of the Protein Society 2006 Jul;15(7):1597-607
Protein science : a publication of the Protein Society 2006 Jul;15(7):1597-607
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ABCG2/CD338 Antibody (BXP-21) [NB100-2177] - Tissue was fixed with formaldehyde, blocked with 3% H2O2 for 5 minutes at 20 C and permeabilized with PBS-Tween; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/200 in PBS-Tween) for 1 hour at 20 C. A biotinylated goat anti-mouse polyclonal IgG was used as the secondary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: ABCG2/CD338 Antibody (BXP-21) [NB100-2177] - Staining in MCF7 human breast cancer cells. Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were then blocked using 10% serum for 2 hours at 25 C.
