V2730

antibody from NSJ Bioreagents

Targeting: MUC3A MUC3

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V2730

Provider

NSJ Bioreagents

Product name

Mucin-3 Antibody

Antibody type

Monoclonal

Antigen

Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the Mucin-3 antibody.

Description

Protein G purified antibody

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

M3.1

Vial size

20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)

Storage

Store the Mucin-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Mapping PAM4 (clivatuzumab), a monoclonal antibody in clinical trials for early detection and therapy of pancreatic ductal adenocarcinoma, to MUC5AC mucin.

Gold DV, Newsome G, Liu D, Goldenberg DM
Molecular cancer 2013 Nov 20;12(1):143

---

Expression profiles of MUC mucins and trefoil factor family (TFF) peptides in the intrahepatic biliary system: physiological distribution and pathological significance.

Sasaki M, Ikeda H, Nakanuma Y
Progress in histochemistry and cytochemistry 2007;42(2):61-110

---

Oncocytic papillary neoplasms of the biliary tract: a clinicopathological, mucin core and Wnt pathway protein analysis of four cases.

Rouzbahman M, Serra S, Adsay NV, Bejarano PA, Nakanuma Y, Chetty R
Pathology 2007 Aug;39(4):413-8

---

Mucin core polypeptide expression in the progression of neoplasia in Barrett's esophagus.

Glickman JN, Blount PL, Sanchez CA, Cowan DS, Wongsurawat VJ, Reid BJ, Odze RD
Human pathology 2006 Oct;37(10):1304-15

---

Selective induction of mucin-3 by hypoxia in intestinal epithelia.

Louis NA, Hamilton KE, Canny G, Shekels LL, Ho SB, Colgan SP
Journal of cellular biochemistry 2006 Dec 15;99(6):1616-27

---

Mucin antibodies - new tools in diagnosis and therapy of cancer.

Wittel UA, Goel A, Varshney GC, Batra SK
Frontiers in bioscience : a journal and virtual library 2001 Oct 1;6:D1296-310

---

Expression of MUC1 and MUC2 and carbohydrate antigen Tn change during malignant transformation of biliary papillomatosis.

Amaya S, Sasaki M, Watanabe Y, Tsui WM, Tsuneyama K, Harada K, Nakanuma Y
Histopathology 2001 Jun;38(6):550-60

---

Expression of the H type 1 blood group antigen during enterocytic differentiation of Caco-2 cells.

Amano J, Oshima M
The Journal of biological chemistry 1999 Jul 23;274(30):21209-16

---

Anti-peptide monoclonal antibodies to intestinal mucin 3.

Apostolopoulos V, Xing PX, McKenzie IF
Journal of gastroenterology and hepatology 1995 Sep-Oct;10(5):555-61

---

Murine immune response to cells transfected with human MUC1: immunization with cellular and synthetic antigens.

Apostolopoulos V, Xing PX, McKenzie IF
Cancer research 1994 Oct 1;54(19):5186-93

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of formalin-fixed, paraffin-embedded human colon carcinoma stained with Mucin-3 antibody (M3.1).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of formalin-fixed, paraffin-embedded human gastric carcinoma stained with Mucin-3 antibody (M3.1).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of formalin-fixed, paraffin-embedded human gastric carcinoma stained with Mucin-3 antibody (M3.1).

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free Mucin-3 Antibody (clone M3.1). Confirmation of Integrity and Purity of the Antibody.