MA5-12350

antibody from Invitrogen Antibodies

Targeting: MUC3A MUC3

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12350

Provider

Invitrogen Antibodies

Product name

MUC3 Monoclonal Antibody (1143/B7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA5-12350 targets Mucin 3 in IHC (P) applications and shows reactivity with Human samples.

Antibody clone number

1143/B7

Concentration

0.2 mg/mL

References

Submitted references

MUC5AC, cytokeratin 20 and HER2 expression and K-RAS mutations within mucinogenic growth in congenital pulmonary airway malformations.

Rossi G, Gasser B, Sartori G, Migaldi M, Costantini M, Mengoli MC, Piccioli S, Cavazza A, Rivasi F
Histopathology 2012 Jun;60(7):1133-43

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Development of human minor salivary glands: expression of mucins according to stage of morphogenesis.

Teshima TH, Ianez RF, Coutinho-Camillo CM, Buim ME, Soares FA, Lourenço SV
Journal of anatomy 2011 Sep;219(3):410-7

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Selective induction of mucin-3 by hypoxia in intestinal epithelia.

Louis NA, Hamilton KE, Canny G, Shekels LL, Ho SB, Colgan SP
Journal of cellular biochemistry 2006 Dec 15;99(6):1616-27

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Mucin 3 showing staining in the membrane and weak cytoplasm of paraffin-embedded human hepatocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Mucin 3 Mouse Monoclonal Antibody (Product # MA5-12350) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.