AP2797a

antibody from Abgent

Targeting: RFC2 A1, RFC40

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2797a

Provider

Abgent

Proper citation

Abgent Cat#AP2797a, RRID:AB_1554198

Product name

RFC2 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-RFC2 Antibody (N-term)(Cat.#AP2797a) in K562 cell line lysates (35ug/lane). RFC2(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term)(Cat.#AP2797a), 1:200 dilution,counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery. (Provided by Hirotaka Ata,University of South Alabama, Dept of Biochem and Mol Biol)"

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RFC2 Antibody (N-term) (Cat. #AP2797a) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50