Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS9272 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- RFC2 (T165) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 150-200 of Human RFC2.
- Description
- Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotidebinding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC2, the third-largest subunit of the RFC complex, exhibits ATP binding which makes it important for both DNA replication and checkpoint function.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of RFC2 (T165) pAb at 1:1000 dilution Lane1:MCF-7 whole cell lysate(48ug) Lane2:The Intestines tissue lysate of Rat(25ug) Lane3:The Intestines tissue lysate of Mouse(35ug) Lane4:The Lung tissue lysate of Rat(39ug) Lane5:The Lung tissue lysate of Mouse(38ug)