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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- NB100-231 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-231, RRID:AB_2178328
- Product name
- Goat Polyclonal RFC2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RFC2 Antibody [NB100-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC2 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RFC2 Antibody [NB100-231] - Whole cell extract (50 ug) from MCF7 and 293T cells (A) or nuclear extract (50 ug) from HeLa cells (B). Antibody used at 0.5 ug/ml (A) and 1 ug/ml (B).
