- MA5-13311 - Invitrogen Antibodies NGFR antibody

Submitted references A phase1 study of stereotactic gene delivery of AAV2-NGF for Alzheimer's disease.

Rafii MS, Baumann TL, Bakay RA, Ostrove JM, Siffert J, Fleisher AS, Herzog CD, Barba D, Pay M, Salmon DP, Chu Y, Kordower JH, Bishop K, Keator D, Potkin S, Bartus RT
Alzheimer's & dementia : the journal of the Alzheimer's Association 2014 Sep;10(5):571-81

The dimensions and characteristics of the subepidermal nerve plexus in human skin--terminal Schwann cells constitute a substantial cell population within the superficial dermis.

Reinisch CM, Tschachler E
Journal of dermatological science 2012 Mar;65(3):162-9

Rac1b increases with progressive tau pathology within cholinergic nucleus basalis neurons in Alzheimer's disease.

Perez SE, Getova DP, He B, Counts SE, Geula C, Desire L, Coutadeur S, Peillon H, Ginsberg SD, Mufson EJ
The American journal of pathology 2012 Feb;180(2):526-40

Low-affinity nerve growth factor receptor (P75 NGFR) as a marker of perineural invasion in malignant melanomas.

Chan MM, Tahan SR
Journal of cutaneous pathology 2010 Mar;37(3):336-43

In situ evidence of involvement of Schwann cells in ulcerative colitis: autocrine and paracrine signaling by A disintegrin and metalloprotease-17-mediated tumor necrosis factor alpha production.

Mosnier JF, Jarry A, Camdessanché JP, Antoine JC, Laboisse CL
Human pathology 2009 Aug;40(8):1159-67

Immunophenotypical pleomorphism expression in sudden cardiac death.

Ceauşu M, Curcă C, Ardeleanu C, Dermengiu D
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2008;49(3):315-20

Immunohistochemical expression patterns of neural and neuroendocrine markers, the neural growth factor receptors and the beta-tubulin II and IV isotypes in human thymus.

Bai M, Papoudou-Bai A, Karatzias G, Doukas M, Goussia A, Stefanaki K, Rontogianni D, Dalavanga Y, Agnantis NJ, Kanavaros P
Anticancer research 2008 Jan-Feb;28(1A):295-303

The activated nerve growth factor receptor p-TrkA is selectively expressed in advanced-stage ovarian carcinoma.

Ødegaard E, Staff AC, Abeler VM, Kopolovic J, Onsrud M, Lazarovici P, Davidson B
Human pathology 2007 Jan;38(1):140-6

Immunohistochemical localization of epithelial rests of Malassez in human periodontal membrane.

Becktor KB, Nolting D, Becktor JP, Kjaer I
European journal of orthodontics 2007 Aug;29(4):350-3

Growth of benign and malignant schwannoma xenografts in severe combined immunodeficiency mice.

Chang LS, Jacob A, Lorenz M, Rock J, Akhmametyeva EM, Mihai G, Schmalbrock P, Chaudhury AR, Lopez R, Yamate J, John MR, Wickert H, Neff BA, Dodson E, Welling DB
The Laryngoscope 2006 Nov;116(11):2018-26

The p75 neurotrophin receptor is widely expressed in conventional papillary thyroid carcinoma.

Rocha AS, Risberg B, Magalhães J, Trovisco V, de Castro IV, Lazarovici P, Soares P, Davidson B, Sobrinho-Simões M
Human pathology 2006 May;37(5):562-8

Differential expression of the neurotrophin receptors p75NTR, TrkA, TrkB and TrkC in human peripheral blood mononuclear cells.

Nassenstein C, Möhring UH, Luttmann W, Virchow JC Jr, Braun A
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2006 Jun;57 Suppl 2:55-63

Origin and ontogeny of mammalian ovarian neurons.

Dees WL, Hiney JK, McArthur NH, Johnson GA, Dissen GA, Ojeda SR
Endocrinology 2006 Aug;147(8):3789-96

Modulation of Fas-induced apoptosis by p75 neurotrophin receptor in a human neuroblastoma cell line.

Giraud S, Lautrette C, Bessette B, Decourt C, Mathonnet M, Jauberteau MO
Apoptosis : an international journal on programmed cell death 2005 Dec;10(6):1271-83

A histological and anatomical profile of pacinian corpuscles from Dupuytren's contracture and the expression of nerve growth factor receptor.

Ehrmantant WR, Graham WP 3rd, Towfighi J, Mackay DR, Ehrlich HP
Plastic and reconstructive surgery 2004 Sep 1;114(3):721-7

A histological and anatomical profile of pacinian corpuscles from Dupuytren's contracture and the expression of nerve growth factor receptor.

Ehrmantant WR, Graham WP 3rd, Towfighi J, Mackay DR, Ehrlich HP
Plastic and reconstructive surgery 2004 Sep 1;114(3):721-7

Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells.

Yagi H, Nomura T, Nakamura K, Yamazaki S, Kitawaki T, Hori S, Maeda M, Onodera M, Uchiyama T, Fujii S, Sakaguchi S
International immunology 2004 Nov;16(11):1643-56

The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma.

Nassenstein C, Braun A, Erpenbeck VJ, Lommatzsch M, Schmidt S, Krug N, Luttmann W, Renz H, Virchow JC Jr
The Journal of experimental medicine 2003 Aug 4;198(3):455-67

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) was performed by loading 25 µg of SH-SY5Y (lane 1), SW480 (lane 2) and PC12 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~66-75 kDa in SH-SY5Y and SW480 cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of NGFR was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of NGFR was performed by loading 30 µg of SH-SY5Y wild type (Lane 1), SH-SY5Y CAS9 (Lane 2), SH-SY5Y NGFR KO (Lane 3) membrane extracts. The blot was probed with Anti-NGFR Monoclonal Antibody (NGFR5), Biotin (Product # MA5-13311) using 1:100 dilution and detected by chemiluminescence with Poly-HRP Streptavidin (Product # N200,1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to NGFR.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-NGFR Monoclonal Antibody (NGFR5), Biotin(Product # MA5-13311) and a 60kDa band corresponding to NGFR was observed across all cell lines tested. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), SW480 (Lane 2), HT-29 (Lane 3), BeWo (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:100 dilution) and detected by chemiluminescence with Poly-HRP Streptavidin (Product # N200,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) (green) showing staining in the membrane of SW480 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) (green) showing staining in the membrane of SW480 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of NGFR was performed using 70% confluent log phase SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NGFR Monoclonal Antibody (NGFR5), Biotin (Product # MA5-13311) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Streptavidin, Alexa Fluor™ 488 conjugate (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membranous and cytosolic localization. Panel e represents HT-29 cells showing no expression of NGFR. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) in PC12 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) in SW480 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Conjugate: Biotin

MA5-13311

Antibody data