12-9400-41

antibody from Invitrogen Antibodies

Targeting: NGFR CD271, p75NTR, TNFRSF16

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12-9400-41

Provider

Invitrogen Antibodies

Product name

CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), PE, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa, type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9. The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271. Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, and microscopy. Applications Tested: This ME20.4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HEK-293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Mouse, Rat, Canine, Porcine, Rabbit

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

ME20.4

Vial size

25 Tests

Concentration

5 µL/Test

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints.

Teunissen M, Ahrens NS, Snel L, Narcisi R, Kamali SA, van Osch GJVM, Meij BP, Mastbergen SC, Sivasubramaniyan K, Tryfonidou MA
Stem cell research & therapy 2022 Sep 5;13(1):457

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In mouse chronic pancreatitis CD25(+)FOXP3(+) regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response.

Glaubitz J, Wilden A, Golchert J, Homuth G, Völker U, Bröker BM, Thiele T, Lerch MM, Mayerle J, Aghdassi AA, Weiss FU, Sendler M
Nature communications 2022 Aug 3;13(1):4502

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Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

Hosokawa H, Koizumi M, Masuhara K, Romero-Wolf M, Tanaka T, Nakayama T, Rothenberg EV
The Journal of experimental medicine 2021 Aug 2;218(8)

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LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice.

Hirano KI, Hosokawa H, Koizumi M, Endo Y, Yahata T, Ando K, Hozumi K
eLife 2021 Aug 12;10

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Notch2 complements Notch1 to mediate inductive signaling that initiates early T cell development.

Romero-Wolf M, Shin B, Zhou W, Koizumi M, Rothenberg EV, Hosokawa H
The Journal of cell biology 2020 Oct 5;219(10)

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Targeting melanoma stem cells with the Vitamin E derivative δ-tocotrienol.

Marzagalli M, Moretti RM, Messi E, Marelli MM, Fontana F, Anastasia A, Bani MR, Beretta G, Limonta P
Scientific reports 2018 Jan 12;8(1):587

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Ibuprofen inhibits survival of bladder cancer cells by induced expression of the p75NTR tumor suppressor protein.

Khwaja F, Allen J, Lynch J, Andrews P, Djakiew D
Cancer research 2004 Sep 1;64(17):6207-13

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Nerve growth factor induces the re-expression of functional androgen receptors and p75(NGFR) in the androgen-insensitive prostate cancer cell line DU145.

Sigala S, Tognazzi N, Rizzetti MC, Faraoni I, Missale C, Bonmassar E, Spano P
European journal of endocrinology 2002 Sep;147(3):407-15

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Bone marrow stroma in humans: anti-nerve growth factor receptor antibodies selectively stain reticular cells in vivo and in vitro.

Cattoretti G, Schiró R, Orazi A, Soligo D, Colombo MP
Blood 1993 Apr 1;81(7):1726-38

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Expression of human nerve growth factor receptor on cells derived from all three germ layers.

Thomson TM, Rettig WJ, Chesa PG, Green SH, Mena AC, Old LJ
Experimental cell research 1988 Feb;174(2):533-9

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Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies.

Ross AH, Grob P, Bothwell M, Elder DE, Ernst CS, Marano N, Ghrist BF, Slemp CC, Herlyn M, Atkinson B
Proceedings of the National Academy of Sciences of the United States of America 1984 Nov;81(21):6681-5

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of 2-day ibuprofen-stimulated HEK-293T cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-CD271 (NGF Receptor) PE (purple histogram). Total viable cells were used for analysis.

Conjugate

Yellow dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chronic pancreatitis leads to pancreatic tissue remodeling. a Flow cytometry analysis of isolated leukocytes from pancreatic tissue showed a significant increase of CCR2 + cells ( p = 0.0032, con n = 7/CP n = 15). b CD11b + labeling identified that also cells of the innate immune system infiltrate into the damaged pancreas ( p < 0.0001, con n = 7/CP n = 15). c Most of these cells were positive for CD206 ( p < 0.0001, con n = 7/CP n = 15) or CD163 ( p < 0.0001, con n = 7/CP n = 15), which characterized them as alternatively activated macrophages. d Labeling of CP tissue for CD206 illustrates their increase in pancreatic tissue of mice. A quantative analysis showed that ~9% of all cells were positive for CD206 in CP tissue ( p < 0.0001, con n = 5/CP n = 9), scale bars represent 20 um. e IL-33 expression of CD206 + macrophages was demonstrated by immunofluorescent labeling, scale bars represent 20 um. f This cytokine is a strong inducer of ILC2s (lin - CD45 + CD127 + CD90 + GATA3 + ) which were also significantly elevated in the pancreas of CP mice ( p = 0.0002, con n = 7/CP n = 15). g The replacement of exocrine pancreas by fibrotic tissue in CP was illustrated by azan blue staining of the pancreas, scale bars represent 50 um. h Flow cytometry analysis of isolated pancreatic cells for PSC marker proteins CD271 ( p < 0.0001, con n = 7/CP n = 15) and GFAP ( p < 0.0001, con n = 7/CP n = 15) identified a significant increase. i Serum collagen levels were significantly elevated in C

Conjugate

Yellow dye