14-9400-82

antibody from Invitrogen Antibodies

Targeting: NGFR CD271, p75NTR, TNFRSF16

Provider product page for 14-9400-82

Western blot

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [7]
Comments [0]
Validations
Immunohistochemistry [1]
Other assay [3]

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Product number: 14-9400-82 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9. The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271. Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This ME20.4 antibody has been tested by flow cytometric analysis of SK-N-SH cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This ME20.4 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity: Human, Mouse, Rat, Canine, Porcine, Rabbit
Host: Mouse
Isotype: IgG
Antibody clone number: ME20.4
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: 4° C

NGFR protein structure - 14-9400-82 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4. piggyBac /Ad-GFP transduces multiple cell types. Tissue from each region of the pig lung (as depicted in Figure 1B ) was sectioned, counterstained with DAPI, and imaged. ( A and B ) piggyBac /Ad-GFP transduces submucosal glands (SMGs) throughout the trachea. ( B and C ) Tissues stained with acetyl-tubulin (red) show that piggyBac /Ad-GFP transduces ciliated (c) and non-ciliated (nc) cells at the airway surface. ( D - F ) piggyBac /Ad-GFP co-localizes with CK5 positive basal cells (red) within submucosal glands ( n = 6). Scale bars for A = 250 mum; B-F = 500 mum. ( G and H ) piggyBac /Ad-GFP transduces basal cells at the airway surface. Arrows indicate GFP positive cells that co-localize with red (G) NGFR or (H) CK14 immunostaining. Images acquired at 63x magnification ( n = 6).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Chronic pancreatitis leads to pancreatic tissue remodeling. a Flow cytometry analysis of isolated leukocytes from pancreatic tissue showed a significant increase of CCR2 + cells ( p = 0.0032, con n = 7/CP n = 15). b CD11b + labeling identified that also cells of the innate immune system infiltrate into the damaged pancreas ( p < 0.0001, con n = 7/CP n = 15). c Most of these cells were positive for CD206 ( p < 0.0001, con n = 7/CP n = 15) or CD163 ( p < 0.0001, con n = 7/CP n = 15), which characterized them as alternatively activated macrophages. d Labeling of CP tissue for CD206 illustrates their increase in pancreatic tissue of mice. A quantative analysis showed that ~9% of all cells were positive for CD206 in CP tissue ( p < 0.0001, con n = 5/CP n = 9), scale bars represent 20 um. e IL-33 expression of CD206 + macrophages was demonstrated by immunofluorescent labeling, scale bars represent 20 um. f This cytokine is a strong inducer of ILC2s (lin - CD45 + CD127 + CD90 + GATA3 + ) which were also significantly elevated in the pancreas of CP mice ( p = 0.0002, con n = 7/CP n = 15). g The replacement of exocrine pancreas by fibrotic tissue in CP was illustrated by azan blue staining of the pancreas, scale bars represent 50 um. h Flow cytometry analysis of isolated pancreatic cells for PSC marker proteins CD271 ( p < 0.0001, con n = 7/CP n = 15) and GFAP ( p < 0.0001, con n = 7/CP n = 15) identified a significant increase. i Serum collagen levels were significantly elevated in C

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Evaluation of the CD marker location in the synovial membrane. Immunohistochemical analysis using DAB (3, 3'-diaminobenzidine, orange/brown staining) was performed to identify the location of CD90, CD44, CD73, CD271, and CD34 expression in the normal (circle) and osteoarthritic (OA, square) synovial membrane. Representative images, containing both fibrous and adipose type synovial membrane, were chosen. The scale bar is set at 50 um. Quantification of the DAB staining was performed with Image ProPlus 6.0 software (Media Cybernetics) in 3 to 6 random regions of interest (ROI) per donor, resulting in a mean percentage of DAB positive staining areas for each donor