MA5-13314

antibody from Invitrogen Antibodies

Targeting: NGFR CD271, p75NTR, TNFRSF16

Western blot

Antibody data

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Validation data

Product number: MA5-13314 - Provider product page
Provider: Invitrogen Antibodies
Product name: NGFR Monoclonal Antibody (NGFR5)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-13314 targets Neurotrophin Receptor /NGF-Receptor (p75NGFR) in FACS, ICC/IF and WB applications and shows reactivity with Feline, Ferret, Human, Rat, Non-human primate, and Rabbit samples. This antibody does not react with rat tissue in Western blot applications. The MA5-13314 immunogen is nGFR from A875 melanoma cells.
Reactivity: Human, Rat, Feline, Rabbit
Host: Mouse
Isotype: IgG
Antibody clone number: NGFR5
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

NGFR protein structure - MA5-13314 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) (green) showing staining in the membrane of SW480 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) (green) showing staining in the membrane of SW480 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) (green) showing staining in the membrane of SW480 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of NGFR was performed using 70% confluent log phase SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NGFR Monoclonal Antibody (NGFR5) (Product # MA5-13314) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membranous and cytosolic localization. Panel e represents HT-29 cells showing no expression of NGFR. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.