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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-41837 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP H3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-41837 detects HNRPH3 in WB and IHC applications. Peptide sequence: DYQGRSTGEA FVQFASKEIA ENALGKHKER IGHRYIEIFR SSRSEIKGFY Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs.
Jimenez L, Yu H, McKenzie AJ, Franklin JL, Patton JG, Liu Q, Weaver AM
Journal of proteome research 2019 Mar 1;18(3):947-959
Journal of proteome research 2019 Mar 1;18(3):947-959
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HNRPH3 was performed by loading 35 µg of MiaPaCa-2 lysate in reducing sample buffer and Page Rµler Protein Ladder (Product # 26616) onto a 10% polyacrylamide gel. Proteins were transferred to PVDF membrane using the wet transfer method. Membrane was blocked in Starting Block Blocking Buffer (Product # 37542). HNRPH3 was detected at approximately 37 kDa using a HNRPH3 polyclonal antibody (Product # PA5-41837) at a dilution of 1:1000 in 5% BSA, followed by a goat anti-rabbit IgG DyLight 800 conjugated secondary antibody (Product # SA5-35571) at a dilution of 1:7000. Data courtesy of Antibody Data Exchange Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Raji cell lysate using an anti-HNRPH3 polyclonal antibody (Product # PA5-41837).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human alveolar tissue using an anti-HNRPH3 polyclonal antibody (Product # PA5-41837).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-HNRPH3 polyclonal antibody (Product # PA5-41837).
